1887

Abstract

A previously unidentified, slowly growing, scotochromogenic species, represented by strain 31118, was discovered during sequence-based reidentification of Korean clinical isolates that had been previously identified as by conventional biochemical tests. Although the 16S rRNA gene sequence of strain 31118 was identical to that of the recently described , phylogenetic analyses based on three independent alternative targets (, and the 16S–23S internal transcribed spacer) showed that it was closely related to but was a distinct phylogenetic entity. Furthermore, the phenetic characteristics of this strain were more similar to those of than to those of . Taken together, these results support the conclusion that this strain represents a novel mycobacterium species, for which the name sp. nov. is proposed. The type strain is 31118 (=DSM 45000 =KCTC 19145).

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2010-02-01
2024-04-18
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References

  1. Butler W. R., Thibert L., Kilburn J. O. 1992; Identification of Mycobacterium avium complex strains and some similar species by high-performance liquid chromatography. J Clin Microbiol 30:2698–2704
    [Google Scholar]
  2. CDC 1999 Mycolic acid pattern standards for HPLC identification of mycobacteria Atlanta, GA: Centers for Disease Control and Prevention;
    [Google Scholar]
  3. Duffey P. S., Guthertz L. S., Evans G. C. 1996; Improved rapid identification of mycobacteria by combining solid-phase extraction with high-performance liquid chromatography analysis of BACTEC cultures. J Clin Microbiol 34:1939–1943
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  5. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  6. Floyd M. M., Guthertz L. S., Silcox V. A., Duffey P. S., Jang Y., Desmond E. P., Crawford J. T., Butler W. R. 1996; Characterization of an SAV organism and proposal of Mycobacterium triplex sp. nov. J Clin Microbiol 34:2963–2967
    [Google Scholar]
  7. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  8. Kent P. T., Kubica G. P. 1985 Public Health Mycobacteriology. Guide for the Level III Laboratory. US Department of Health and Human Services publication Atlanta, GA: Centers for Disease Control and Prevention;
    [Google Scholar]
  9. Kim B. J., Lee S. H., Lyu M. A., Kim S. J., Bai G. H., Chae G. T., Kim E. C., Cha C. Y., Kook Y. H. 1999; Identification of mycobacterial species by comparative sequence analysis of the RNA polymerase gene ( rpoB ). J Clin Microbiol 37:1714–1720
    [Google Scholar]
  10. Kim H., Kim S. H., Shim T. S., Kim M. N., Bai G. H., Park Y. G., Lee S. H., Chae G. T., Cha C. Y. other authors 2005; Differentiation of Mycobacterium species by analysis of the heat-shock protein 65 gene ( hsp65 ). Int J Syst Evol Microbiol 55:1649–1656 [CrossRef]
    [Google Scholar]
  11. Kirschner P., Springer B., Vogel U., Meier A., Wrede A., Kiekenbeck M., Bange F. C., Bottger E. C. 1993; Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory. J Clin Microbiol 31:2882–2889
    [Google Scholar]
  12. Kumar S., Dudley J., Nei M., Tamura K. 2008; mega: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 9:299–306 [CrossRef]
    [Google Scholar]
  13. Mohamed A. M., Iwen P. C., Tarantolo S., Hinrichs S. H. 2004; Mycobacterium nebraskense sp. nov., a novel slowly growing scotochromogenic species. Int J Syst Evol Microbiol 54:2057–2060 [CrossRef]
    [Google Scholar]
  14. Mun H. S., Kim H. J., Oh E. J., Kim H., Bai G. H., Yu H. K., Park Y. G., Cha C. Y., Kook Y. H., Kim B. J. 2007; Mycobacterium seoulense sp. nov., a slowly growing scotochromogenic species. Int J Syst Evol Microbiol 57:594–599 [CrossRef]
    [Google Scholar]
  15. Mun H. S., Park J. H., Kim H., Yu H. K., Park Y. G., Cha C. Y., Kook Y. H., Kim B. J. 2008; Mycobacterium senuense sp. nov., a slowly growing, non-chromogenic species closely related to the Mycobacterium terrae complex. Int J Syst Evol Microbiol 58:641–646 [CrossRef]
    [Google Scholar]
  16. Rogall T., Wolters J., Flohr T., Bottger E. C. 1990; Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium . Int J Syst Bacteriol 40:323–330 [CrossRef]
    [Google Scholar]
  17. Roth A., Fischer M., Hamid M. E., Michalke S., Ludwig W., Mauch H. 1998; Differentiation of phylogenetically related slowly growing mycobacteria based on 16S–23S rRNA gene internal transcribed spacer sequences. J Clin Microbiol 36:139–147
    [Google Scholar]
  18. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  19. Springer B., Stockman L., Teschner K., Roberts G. D., Bottger E. C. 1996; Two-laboratory collaborative study on identification of mycobacteria: molecular versus phenotypic methods. J Clin Microbiol 34:296–303
    [Google Scholar]
  20. Turenne C. Y., Tschetter L., Wolfe J., Kabani A. 2001; Necessity of quality-controlled 16S rRNA gene sequence databases: identifying nontuberculous Mycobacterium species. J Clin Microbiol 39:3637–3648 [CrossRef]
    [Google Scholar]
  21. Turenne C. Y., Cook V. J., Burdz T. V., Pauls R. J., Thibert L., Wolfe J. N., Kabani A. 2004; Mycobacterium parascrofulaceum sp. nov., novel slowly growing, scotochromogenic clinical isolates related to Mycobacterium simiae . Int J Syst Evol Microbiol 54:1543–1551 [CrossRef]
    [Google Scholar]
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