@article{mbs:/content/journal/ijsem/10.1099/ijs.0.012484-0, author = "Iino, Takao and Mori, Koji and Uchino, Yoshihito and Nakagawa, Tatsunori and Harayama, Shigeaki and Suzuki, Ken-ichiro", title = "Ignavibacterium album gen. nov., sp. nov., a moderately thermophilic anaerobic bacterium isolated from microbial mats at a terrestrial hot spring and proposal of Ignavibacteria classis nov., for a novel lineage at the periphery of green sulfur bacteria", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2010", volume = "60", number = "6", pages = "1376-1382", doi = "https://doi.org/10.1099/ijs.0.012484-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.012484-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "ML, maximum-likelihood", keywords = "NJ, neighbour-joining", keywords = "MP, maximum-parsimony", abstract = "A moderately thermophilic chemoheterotrophic bacterium, strain Mat9-16T, was isolated from microbial mats developed in hot spring water streams from Yumata, Nagano, Japan. Cells of strain Mat9-16T were strictly anaerobic, Gram-stain-negative, non-sporulating, non-motile and short to long rods (2.0–15.5 μm in length). Strain Mat9-16T grew fermentatively with optimum growth at 45 °C, pH 7.0–7.5 and 1 % NaCl (w/v). Phylogenetic analysis based on the 16S rRNA gene revealed that strain Mat9-16T was affiliated with an uncultivated lineage, and the nearest cultivated neighbours were green sulfur bacteria belonging to the class Chlorobea with 77–83 % sequence similarity. However, strain Mat9-16T could not grow phototrophically and did not possess light-harvesting structures, morphologically and genetically, such as the chlorosomes of green sulfur bacteria. On the basis of phenotypic features and phylogenetic position, a novel genus and species are proposed for strain Mat9-16T, to be named Ignavibacterium album gen. nov., sp. nov. (=NBRC 101810T =DSM 19864T). We also propose to place the cultivated bacterial lineage accommodating the sole representative Mat9-16T in a novel class, Ignavibacteria classis nov. In addition, we present a formal description of the phylum-level taxon ‘Chlorobi’ as Chlorobi phyl. nov.", }