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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 60, Issue 6

gen. nov., sp. nov., a moderately thermophilic anaerobic bacterium isolated from microbial mats at a terrestrial hot spring and proposal of classis nov., for a novel lineage at the periphery of green sulfur bacteria Free

Abstract

A moderately thermophilic chemoheterotrophic bacterium, strain Mat9-16, was isolated from microbial mats developed in hot spring water streams from Yumata, Nagano, Japan. Cells of strain Mat9-16 were strictly anaerobic, Gram-stain-negative, non-sporulating, non-motile and short to long rods (2.0–15.5 μm in length). Strain Mat9-16 grew fermentatively with optimum growth at 45 °C, pH 7.0–7.5 and 1 % NaCl (w/v). Phylogenetic analysis based on the 16S rRNA gene revealed that strain Mat9-16 was affiliated with an uncultivated lineage, and the nearest cultivated neighbours were green sulfur bacteria belonging to the class with 77–83 % sequence similarity. However, strain Mat9-16 could not grow phototrophically and did not possess light-harvesting structures, morphologically and genetically, such as the chlorosomes of green sulfur bacteria. On the basis of phenotypic features and phylogenetic position, a novel genus and species are proposed for strain Mat9-16, to be named gen. nov., sp. nov. (=NBRC 101810 =DSM 19864). We also propose to place the cultivated bacterial lineage accommodating the sole representative Mat9-16 in a novel class, classis nov. In addition, we present a formal description of the phylum-level taxon as phyl. nov.

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Keyword(s): ML, maximum-likelihood , MP, maximum-parsimony and NJ, neighbour-joining
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2010-06-01
2024-04-19
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Ignavibacterium album gen. nov., sp. nov., a moderately thermophilic anaerobic bacterium isolated from microbial mats at a terrestrial hot spring and proposal of Ignavibacteria classis nov., for a novel lineage at the periphery of green sulfur bacteria
Int J Syst Evol Microbiol 60, 1376 (2010); https://doi.org/10.1099/ijs.0.012484-0
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