1887

Abstract

Gram-positive, non-spore-forming rods were isolated from a human osteo-articular sample (strain 7400942). Based on cellular morphology and the results of biochemical analysis, this strain was tentatively identified as a novel species of the genus . Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that the bacterium was closely related to the type strain of (96.9 % 16S rRNA gene sequence similarity). A comparison of biochemical traits showed that strain 7400942 was distinct from in a number of characteristics, i.e. in contrast with , strain 7400942 was negative for nitrate reduction and for -galactosidase, -glucosidase and alanine arylamidase activities, it was positive for acid production from -acetylglucosamine, melezitose and glycogen, and it was negative for acid production from turanose. Matrix-assisted laser-desorption/ionization time-of-flight MS protein analysis confirmed that strain 7400942 represents a novel species, as scores obtained for its spectra were significant (>2.2) only with strain 7400942. On the basis of phenotypic data and phylogenetic inference, it is proposed that this strain should be designated sp. nov.; the type strain is strain 7400942 (=CSUR P35=CCUG 55928).

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2010-07-01
2024-03-29
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References

  1. Acevedo F., Baudrand R., Letelier L. M., Gaete P. 2008; Actinomycosis: a great pretender. Case reports of unusual presentations and a review of the literature. Int J Infect Dis 12:358–362 [CrossRef]
    [Google Scholar]
  2. An D., Cai S., Dong X. 2006; Actinomyces ruminicola sp. nov., isolated from cattle rumen. Int J Syst Evol Microbiol 56:2043–2048 [CrossRef]
    [Google Scholar]
  3. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
    [Google Scholar]
  4. Funke G., von Graevenitz A., Clarridge J. E. III, Bernard K. E. 1997; Clinical microbiology of coryneform bacteria. Clin Microbiol Rev 10:125–159
    [Google Scholar]
  5. Gouriet F., Fenollar F., Patrice J. Y., Drancourt M., Raoult D. 2005; Use of shell-vial cell culture assay for isolation of bacteria from clinical specimens: 13 years of experience. J Clin Microbiol 43:4993–5002 [CrossRef]
    [Google Scholar]
  6. Hall V. 2008; Actinomyces – gathering evidence of human colonization and infection. Anaerobe 14:1–7 [CrossRef]
    [Google Scholar]
  7. Hall V., Talbot P. R., Stubbs S. L., Duerden B. I. 2001; Identification of clinical isolates of Actinomyces species by amplified 16S ribosomal DNA restriction analysis. J Clin Microbiol 39:3555–3562 [CrossRef]
    [Google Scholar]
  8. Hall V., Collins M. D., Hutson R. A., Inganas E., Falsen E., Duerden B. I. 2003a; Actinomyces oricola sp. nov., from a human dental abscess. Int J Syst Evol Microbiol 53:1515–1518 [CrossRef]
    [Google Scholar]
  9. Hall V., Collins M. D., Lawson P. A., Falsen E., Duerden B. I. 2003b; Actinomyces nasicola sp. nov., isolated from a human nose. Int J Syst Evol Microbiol 53:1445–1448 [CrossRef]
    [Google Scholar]
  10. Hall V., Collins M. D., Lawson P. A., Falsen E., Duerden B. I. 2005; Actinomyces dentalis sp. nov., from a human dental abscess. Int J Syst Evol Microbiol 55:427–431 [CrossRef]
    [Google Scholar]
  11. Kerttula A. M., Carlson P., Sarkonen N., Hall V., Könönen E. 2005; Enzymatic/biochemical analysis of Actinomyces with commercial test kits with an emphasis on newly described species. Anaerobe 11:99–108 [CrossRef]
    [Google Scholar]
  12. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  13. Mellmann A., Cloud J., Maier T., Keckevoet U., Ramminger I., Iwen P., Dunn J., Hall G., Wilson D. other authors 2008; Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria. J Clin Microbiol 46:1946–1954 [CrossRef]
    [Google Scholar]
  14. Miller P. H., Wiggs L. S., Miller J. M. 1995; Evaluation of API An-IDENT and RapID ANA II systems for identification of Actinomyces species from clinical specimens. J Clin Microbiol 33:329–330
    [Google Scholar]
  15. Pascual C., Foster G., Falsen E., Bergström K., Greko C., Collins M. D. 1999; Actinomyces bowdenii sp. nov., isolated from canine and feline clinical specimens. Int J Syst Bacteriol 49:1873–1877 [CrossRef]
    [Google Scholar]
  16. Rothschild B., Naples V., Barbian L. 2006; Bone manifestations of actinomycosis. Ann Diagn Pathol 10:24–27 [CrossRef]
    [Google Scholar]
  17. Saitou N., Nei M. 1987; The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  18. Santala A. M., Sarkonen N., Hall V., Carlson P., Jousimies-Somer H., Könönen E. 2004; Evaluation of four commercial test systems for identification of Actinomyces and some closely related species. J Clin Microbiol 42:418–420 [CrossRef]
    [Google Scholar]
  19. Song Y. 2005; PCR-based diagnostics for anaerobic infections. Anaerobe 11:79–91 [CrossRef]
    [Google Scholar]
  20. Stîngu C. S., Rodloff A. C., Jentsch H., Schaumann R., Eschrich K. 2008; Rapid identification of oral anaerobic bacteria cultivated from subgingival biofilm by MALDI-TOF-MS. Oral Microbiol Immunol 23:372–376 [CrossRef]
    [Google Scholar]
  21. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  22. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
    [Google Scholar]
  23. Woo P. C., Ng K. H. L., Lau S. K. P., Yip K. T., Fung A. M. Y., Leung K. W., Tam D. M. W., Que T. L., Yuen K. Y. 2003; Usefulness of the MicroSeq 500 16S ribosomal DNA-based bacterial identification system for identification of clinically significant bacterial isolates with ambiguous biochemical profiles. J Clin Microbiol 41:1996–2001 [CrossRef]
    [Google Scholar]
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