1887

Abstract

Two yeast strains (FMCC Y-1 and FMCC Y-2) were recovered during a survey of the yeast biota associated with fermenting black olives, collected from an olive tree ( L. cv. ‘Conservolea’) orchard in Central Greece. Phylogenetic analysis based on rRNA gene sequences (18S, 26S, and 5.8S–ITS) indicated that the two strains represent a separate species within the clade, in close relation to NRRL Y-27703. Electrophoretic karyotyping and physiological analysis support the affiliation of the two strains to a novel species as sp. nov. The novel strains are conspecific with two sp. strains previously isolated from the Mid-Atlantic Ridge hydrothermal fields [Gadanho & Sampaio (2005). , 408–417], indicating that sp. nov. may occupy diverse ecological niches. FMCC Y-1 (=CBS 11171 =ATCC MYA-4568) is the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.012955-0
2010-05-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/5/1219.html?itemId=/content/journal/ijsem/10.1099/ijs.0.012955-0&mimeType=html&fmt=ahah

References

  1. Arroyo-López F. N., Querol A., Bautista-Gallego J., Garrido-Fernández A. 2008; Role of yeasts in table olive production. Int J Food Microbiol 128:189–196 [CrossRef]
    [Google Scholar]
  2. Bai F.-Y., Liang H.-Y., Jia J.-H. 2000; Taxonomic relationships among the taxa in the Candida guilliermondii complex, as revealed by comparative electrophoretic karyotyping. Int J Syst Evol Microbiol 50:417–422 [CrossRef]
    [Google Scholar]
  3. Burke D., Dawson D., Stearns T. 2000; Yeast genomic DNA: glass bead preparation. In Methods in Yeast Genetics, a Cold Spring Harbor Laboratory Course Manual pp 113–114 Cold Spring Harbor NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  4. Gadanho M., Sampaio J. P. 2005; Occurrence and diversity of yeasts in the Mid-Atlantic Ridge hydrothermal fields near the Azores Archipelago. Microb Ecol 50:408–417 [CrossRef]
    [Google Scholar]
  5. Imanishi Y., Ueda-Nishimura K., Mikata K. 2007; Two new species of Kazachstania that form ascospores connected by a belt-like intersporal body: Kazachstania zonata and Kazachstania gamospora . FEMS Yeast Res 7:330–338 [CrossRef]
    [Google Scholar]
  6. Kurtzman C. P., Robnett C. J. 1998; Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie Van Leeuwenhoek 73:331–371 [CrossRef]
    [Google Scholar]
  7. Meyer S. A., Payne R. W., Yarrow D. 1998; Candida Berkhout. In The Yeasts, a Taxonomic Study , 4th edn. pp 454–573 Edited by Kurtzman C. P., Fell J. W. Amsterdam: Elsevier;
    [Google Scholar]
  8. Nisiotou A. A., Gibson G. R. 2005; Isolation of culturable yeasts from market wines and evaluation of the 5.8S-ITS rDNA sequence analysis for identification purposes. Lett Appl Microbiol 41:454–463 [CrossRef]
    [Google Scholar]
  9. Nychas G.-J. E., Panagou E. Z., Parker M. L., Waldron K. W., Tassou C. C. 2002; Microbial colonization of naturally black olives during fermentation and associated biochemical activities in the cover brine. Lett Appl Microbiol 34:173–177 [CrossRef]
    [Google Scholar]
  10. Suh S.-O., Nguyen N. H., Blackwell M. 2005; Nine new Candida species near C. membranifaciens isolated from insects. Mycol Res 109:1045–1056 [CrossRef]
    [Google Scholar]
  11. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  12. Thompson J. D., Higgins D. G., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. J. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  13. Ueda-Nishimura K., Mikata K. 1999; A new yeast genus, Tetrapisispora gen. nov.: Tetrapisispora iriomotensis sp.nov., Tetrapisispora nanseiensis sp. nov. and Tetrapisispora arboricola sp. nov.,from the Nansei Islands, and reclassification of Kluyveromyces phaffii (van der Walt) van der Walt as Tetrapisispora phaffii comb. nov. Int J Syst Bacteriol 49:1915–1924 [CrossRef]
    [Google Scholar]
  14. Yarrow D. 1998; Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study, , 4th edn. pp 77–100 Edited by Kurtzman C. P, Fell J. W. Amsterdam: Elsevier;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.012955-0
Loading
/content/journal/ijsem/10.1099/ijs.0.012955-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error