1887

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Volume 60, Issue 6

sp. nov., isolated from soil Free

Abstract

A Gram-negative, non-motile, pale-yellow, rod-shaped bacterial strain, DS-42, was isolated from a soil in Korea and its taxonomic position was investigated by a polyphasic study. Strain DS-42 grew optimally at 25 °C and pH 7.0–8.0. Strain DS-42 did not form nodules on three different legumes, and the and genes were also not detected by PCR. Strain DS-42 contained Q-10 as the predominant ubiquinone. The major cellular fatty acid was C 7. The DNA G+C content was 60.8 mol%. Phylogenetic analyses based on 16S rRNA, and gene sequences showed that strain DS-42 belonged to the genus . Strain DS-42 showed 16S rRNA gene sequence similarity of 94.1–97.7 % to the type strains of recognized species. DNA–DNA relatedness between strain DS-42 and the type strains of , , and was 13–19 %, indicating that strain DS-42 was distinct from them genetically. Strain DS-42 can also be differentiated from these four phylogenetically related species by various phenotypic properties. On the basis of phenotypic properties, phylogenetic distinctiveness and genetic data, strain DS-42 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DS-42 (=KCTC 12873 =JCM 14591).

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2010-06-01
2024-04-18
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References

  1. Berge O., Lodhi A., Brandelet G., Santaella C., Roncato M.-A., Christen R., Heulin T., Achouak W. 2009; Rhizobium alamii sp. nov., an exopolysaccharide-producing species isolated from legume and non-legume rhizospheres. Int J Syst Evol Microbiol 59:367–372 [CrossRef]
     [Google Scholar]
  2. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
     [Google Scholar]
  3. Euzéby J. P. 1997; List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. Int J Syst Bacteriol 47:590–592 http://www.bacterio.cict.fr [CrossRef]
     [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
     [Google Scholar]
  5. Frank B. 1889; Über die Pilzsymbiose der Leguminosen. Ber Dtsch Bot Ges 7:332–346 (in German)
    [Google Scholar]
  6. García-Fraile P., Rivas R., Willems A., Peix A., Martens M., Martínez-Molina E., Mateos P. F., Velázquez E. 2007; Rhizobium cellulosilyticum sp. nov., isolated from sawdust of Populus alba . Int J Syst Evol Microbiol 57:844–848 [CrossRef]
     [Google Scholar]
  7. Gu C. T., Wang E. T., Tian C. F., Han T. X., Chen W. F., Sui X. H., Chen W. X. 2008; Rhizobium miluonense sp. nov., a symbiotic bacterium isolated from Lespedeza root nodules. Int J Syst Evol Microbiol 58:1364–1368 [CrossRef]
     [Google Scholar]
  8. Han T. X., Wang E. T., Wu L. J., Chen W. F., Gu J. G., Gu C. T., Tian C. F., Chen W. X. 2008; Rhizobium multihospitium sp. nov., isolated from multiple legume species native of Xinjiang, China. Int J Syst Evol Microbiol 58:1693–1699 [CrossRef]
     [Google Scholar]
  9. Hunter W. J., Kuykendall L. D., Manter D. K. 2007; Rhizobium selenireducens sp. nov. a selenite-reducing α - Proteobacteria isolated from a bioreactor. Curr Microbiol 55:455–460 [CrossRef]
     [Google Scholar]
  10. Hunter W. J., Kuykendall L. D., Manter D. K. 2008; Rhizobium selenireducens sp. nov. In List of New Names and New Combinations Previously Effectively, but not Validly, Published , Validation List no. 121. Int J Syst Evol Microbiol 58:1057 [CrossRef]
     [Google Scholar]
  11. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
     [Google Scholar]
  12. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
     [Google Scholar]
  13. Lee K.-B., Liu C.-T., Anzai Y., Kim H., Aono T., Oyaizu H. 2005; The hierarchical system of the ‘ Alphaproteobacteria ’: description of Hyphomonadaceae fam.nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov. Int J Syst Evol Microbiol 55:1907–1919 [CrossRef]
     [Google Scholar]
  14. Peng G., Yuan Q., Li H., Zhang W., Tan Z. 2008; Rhizobium oryzae sp. nov., isolated from the wild rice Oryza alta . Int J Syst Evol Microbiol 58:2158–2163 [CrossRef]
     [Google Scholar]
  15. Poly F., Monrozier L. J., Bally R. 2001; Improvement in the RFLP procedure for studying the diversity of nifH genes in communities of nitrogen fixers in soil. Res Microbiol 152:95–103 [CrossRef]
     [Google Scholar]
  16. Quan Z.-X., Bae H.-S., Baek J.-H., Chen W.-F., Im W.-T., Lee S.-T. 2005; Rhizobium daejeonense sp. nov., isolated from a cyanide treatment bioreactor. Int J Syst Evol Microbiol 55:2543–2549 [CrossRef]
     [Google Scholar]
  17. Ramírez-Bahena M. H., García-Fraile P., Peix A., Valverde A., Rivas R., Igual J. M., Mateos P. F., Martínez-Molina E., Velázquez E. 2008; Revision of the taxonomic status of the species Rhizobium leguminosarum (Frank 1879) Frank 1889AL, Rhizobium phaseoli Dangeard 1926AL and Rhizobium trifolii Dangeard 1926AL. R. trifolii is a later synonym of R. leguminosarum . Reclassification of the strain R.leguminosarum DSM 30132 (=NCIMB 11478) as Rhizobium pisi sp. nov. Int J Syst Evol Microbiol 58:2484–2490 [CrossRef]
     [Google Scholar]
  18. Rivas R., Velázquez E., Willems A., Vizcaíno N., Subba-Rao N. S., Mateos P. F., Gillis M., Dazzo F. B., Martínez-Molina E. 2002; A new species of Devosia that forms a unique nitrogen-fixing root-nodule symbiosis with the aquatic legume Neptunia natans (L.f.) Druce. Appl Environ Microbiol 68:5217–5222 [CrossRef]
     [Google Scholar]
  19. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids . Technical Note no: 101 Newark, DE: MIDI;
     [Google Scholar]
  20. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
     [Google Scholar]
  21. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
     [Google Scholar]
  22. Tian C. F., Wang E. T., Wu L. J., Han T. X., Chen W. F., Gu C. T., Gu J. G., Chen W. X. 2008; Rhizobium fabae sp. nov., a bacterium that nodulates Vicia faba . Int J Syst Evol Microbiol 58:2871–2875 [CrossRef]
     [Google Scholar]
  23. Valverde A., Igual J. M., Peix A., Cervantes E., Velázquez E. 2006; Rhizobium lusitanum sp. nov. a bacterium that nodulates Phaseolus vulgaris . Int J Syst Evol Microbiol 56:2631–2637 [CrossRef]
     [Google Scholar]
  24. Wang E. T., van Berkum P., Beyene D., Sui X. H., Dorado O., Chen W. X., Martínez-Romero E. 1998; Rhizobium huautlense sp. nov., a symbiont of Sesbania herbacea that has a close phylogenetic relationship with Rhizobium galegae . Int J Syst Bacteriol 48:687–699 [CrossRef]
     [Google Scholar]
  25. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
     [Google Scholar]
  26. Wei G. H., Tan Z. Y., Zhu M. E., Wang E. T., Han S. Z., Chen W. X. 2003; Characterization of rhizobia isolated from legume species within the genera Astragalus and Lespedeza grown in the Loess Plateau of China and description of Rhizobium loessense sp. nov. Int J Syst Evol Microbiol 53:1575–1583 [CrossRef]
     [Google Scholar]
  27. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
     [Google Scholar]
  28. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
     [Google Scholar]
  29. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H. 2003; Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53:53–57 [CrossRef]
     [Google Scholar]
  30. Young J. M., Kuykendall L. D., Martínez-Romero E., Kerr A., Sawada H. 2001; A revision of Rhizobium Frank 1889, with an emended description of the genus, and the inclusion of all species of Agrobacterium Conn 1942 and Allorhizobium undicola de Lajudie et al. 1998 as new combinations:Rhizobium radiobacter , R. rhizogenes , R. rubi , R.undicola and R. vitis . Int J Syst Evol Microbiol 51:89–103
     [Google Scholar]
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Rhizobium soli sp. nov., isolated from soil
Int J Syst Evol Microbiol 60, 1387 (2010); https://doi.org/10.1099/ijs.0.013094-0
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