1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 60, Issue 4

sp. nov., a moderately thermophilic alphaproteobacterium isolated from hot spring sediment Free

Abstract

A moderately thermophilic bacterial strain (HT4) isolated from a hot spring sediment was characterized phenotypically and phylogenetically. Cells were Gram-negative, aerobic, non-sporulating, rod-shaped and motile by means of a single polar flagellum. Both oxidase and catalase activities were positive. Heterotrophic growth was observed at pH 6.0–8.5 and at 20–50 °C; optimum growth occurred at pH 7.5–8.0 and 37–42 °C. The major cellular fatty acids were C 3-OH, C 3-OH, C 2-OH, C 7 and C cyclo 8. The DNA G+C content of strain HT4 was 67.8 mol%.16S rRNA gene sequence analysis indicated that strain HT4 clustered within the radiation of the genus and showed 99.0 % similarity with CCUG 54519 and 96 % similarity with DSM 6462. However, levels of DNA–DNA relatedness between strain HT4 and CCUG 54519 and DSM 6462 were 52 and 20 %, respectively. On the basis of the phenotypic, physiological and chemotaxonomic data, 16S rRNA gene sequence analysis and DNA–DNA hybridization results, strain HT4 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HT4 (=DSM 18167=JCM 14988).

  • Published Online:
SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.013466-0
2010-04-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/4/861.html?itemId=/content/journal/ijsem/10.1099/ijs.0.013466-0&mimeType=html&fmt=ahah

References

  1. Alarico S., Rainey F. A., Empadinhas N., Schumann P., Nobre M. F., da Costa M. S. 2002; Rubritepida flocculans gen. nov., sp. nov., a new slightly thermophilic member of the alpha-1 subclass of the Proteobacteria. Syst Appl Microbiol 25:198–206 [CrossRef]
     [Google Scholar]
  2. Berglund F., Sorbo B. H. 1960; Turbidimetric analysis of inorganic sulfate in serum, plasma and urine. Scand J Clin Lab Invest 12:147–153 [CrossRef]
     [Google Scholar]
  3. Das S. K., Mishra A. K., Tindall B. J., Rainey F. A., Stackebrandt E. 1996; Oxidation of thiosulfate by a new bacterium, Bosea thiooxidans (strain BI-42) gen. nov., sp. nov.: analysis of phylogeny based on chemotaxonomy and 16S ribosomal DNA sequencing. Int J Syst Bacteriol 46:981–987 [CrossRef]
     [Google Scholar]
  4. Denner E. M. B., Kolari M., Hoornstra D., Tsitko I., Kämpfer P., Busse H.-J., Salkinoja-Salonen M. 2006; Rubellimicrobium thermophilum gen. nov., sp. nov., a red-pigmented, moderately thermophilic bacterium isolated from coloured slime deposits in paper machines. Int J Syst Evol Microbiol 56:1355–1362 [CrossRef]
     [Google Scholar]
  5. Donachie S. P., Bowman J. P., Alam M. 2006; Nesiotobacter exalbescens gen. nov., sp. nov., a moderately thermophilic alphaproteobacterium from an Hawaiian hypersaline lake. Int J Syst Evol Microbiol 56:563–567 [CrossRef]
     [Google Scholar]
  6. Egli T. W., Auling G. 2005; Genus Chelatococcus . In Bergey's Manual of Systematic Bacteriology , Part C vol 2, 2nd edn. pp 433–437 Edited by Brenner D. J., Krieg N. R., Stanley J. T. New York: Springer;
     [Google Scholar]
  7. Egli T. W., Weilenmann H.-U., El-Banna T., Auling G. 1988; Gram-negative, aerobic, nitrilotriacetate-utilizing bacteria from wastewater and soil. Syst Appl Microbiol 10:297–305 [CrossRef]
     [Google Scholar]
  8. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
     [Google Scholar]
  9. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
     [Google Scholar]
  10. Ghosh D., Bal B., Kashyap V. K., Pal S. 2003; Molecular phylogenetic exploration of bacterial diversity in a Bakreshwar (India) hot spring and culture of Shewanella -related thermophiles. Appl Environ Microbiol 69:4332–4336 [CrossRef]
     [Google Scholar]
  11. Gleen H., Quastel J. H. 1952; Sulfur metabolism in soil. Appl Microbiol 1:70–77
     [Google Scholar]
  12. Gupta M. L., Narain H., Saxena V. K. 1975; Geochemistry of thermal waters from various geothermal provinces of India. In Proceedings of the Grenoble Symposium of the International Association of Hydrological Sciences pp 47–58 Wallingford, UK: IAHS Press, Institute of Hydrology;
     [Google Scholar]
  13. Johnson D. B., Stallwood B., Kimura S., Hallberg K. B. 2006; Isolation and characterization of Acidicaldus organivorus , gen. nov., sp. nov.: a novel sulfur-oxidizing, ferric iron-reducing thermo-acidophilic heterotrophic Proteobacterium. Arch Microbiol 185:212–221 [CrossRef]
     [Google Scholar]
  14. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp 21–132 Edited by Munro H. N. New York: Academic Press;
     [Google Scholar]
  15. Kanso S., Patel B. K. 2004; Phenylobacterium lituiforme sp. nov., a moderately thermophilic bacterium from a subsurface aquifer, and emended description of the genus Phenylobacterium . Int J Syst Evol Microbiol 54:2141–2146 [CrossRef]
     [Google Scholar]
  16. Kelly D. P., Chambers L. A., Trudinger P. A. 1969; Cyanolysis and spectrophotometric estimation of trithionate in mixture with thiosulfate and tetrathionate. Anal Chem 41:898–901 [CrossRef]
     [Google Scholar]
  17. Kuykendall L. D., Roy M. D., O’Neill J. J., Devine T. E. 1988; Fatty acids, antibiotic resistance and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . Int J Syst Bacteriol 38:358–361 [CrossRef]
     [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
     [Google Scholar]
  19. Panda S. K., Jyoti V., Bhadra B., Nayak K. C., Shivaji S., Rainey F. A., Das S. K. 2009; Thiomonas bhubaneswarensis sp. nov., an obligately mixotrophic, moderately thermophilic, thiosulfate-oxidizing bacterium. Int J Syst Evol Microbiol 59:2171–2175 [CrossRef]
     [Google Scholar]
  20. Rainey F. A., Silva J., Nobre M. F., Silva M. T., da Costa M. S. 2003; Porphyrobacter cryptus sp. nov., a novel slightly thermophilic, aerobic, bacteriochlorophyll a -containing species. Int J Syst Evol Microbiol 53:35–41 [CrossRef]
     [Google Scholar]
  21. Sharma D. P., Thomas C., Hall R. H., Levine M. M., Attridge S. R. 1989; Significance of toxin coregulated pili as protective antigens of Vibrio cholerae in the infant mouse model. Vaccine 7:451–456 [CrossRef]
     [Google Scholar]
  22. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849
     [Google Scholar]
  23. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
     [Google Scholar]
  24. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
     [Google Scholar]
  25. Wayne L. G., Good R. C., Krichevsky M. I., Blacklock Z., David H. L., Dawson D., Gross W., Hawkins J., Levy-Frebault V. V. other authors 1991; Fourth report of the cooperative, open-ended study of slowly growing mycobacteria by the International Working Group on Mycobacterial Taxonomy. Int J Syst Bacteriol 41:463–472 [CrossRef]
     [Google Scholar]
  26. Yoon J. H., Kang S. J., Im W. T., Lee S. T., Oh T. K. 2008; Chelatococcus daeguensis sp. nov., isolated from wastewater of a textile dye works, and emended description of the genus Chelatococcus . Int J Syst Evol Microbiol 58:2224–2228 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.013466-0
Loading
Chelatococcus sambhunathii sp. nov., a moderately thermophilic alphaproteobacterium isolated from hot spring sediment
Int J Syst Evol Microbiol 60, 861 (2010); https://doi.org/10.1099/ijs.0.013466-0
/content/journal/ijsem/10.1099/ijs.0.013466-0
/content/journal/ijsem/10.1099/ijs.0.013466-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error