RT Journal Article SR Electronic(1) A1 Guo, Xin-Qiang A1 Li, Rong A1 Zheng, Liu-Qiang A1 Lin, Dong-Qing A1 Sun, Ji-Quan A1 Li, Shun-Peng A1 Li, Wen-Jun A1 Jiang, Jian-DongYR 2010 T1 Jeotgalicoccus huakuii sp. nov., a halotolerant bacterium isolated from seaside soil JF International Journal of Systematic and Evolutionary Microbiology, VO 60 IS 6 SP 1307 OP 1310 DO https://doi.org/10.1099/ijs.0.013623-0 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-positive, oxidase- and catalase-positive, non-motile, non-spore-forming, halotolerant, coccoid bacterium, designated strain NY-2T, was isolated from a seaside soil sample from Shandong Province, China. Strain NY-2T was able to grow in the presence of 0–23 % (w/v) NaCl and at pH 4.5–10.0 and 5–42 °C; optimum growth was observed with 3–8 % (w/v) total salts and at pH 6.5–8.0 and 28–37 °C. Chemotaxonomic analyses, including fatty acid profiles, menaquinones and polar lipids, supported the affiliation of strain NY-2T to the genus Jeotgalicoccus. The predominant menaquinone of strain NY-2T was menaquinone 7 (MK-7) (100 %) and the major cellular fatty acids were iso-C15 : 0 (49.0 %) and anteiso-C15 : 0 (19.6 %). Cellular polar lipids were phosphatidylglycerol, diphosphatidylglycerol and several unidentified phospholipids. The DNA G+C content of strain NY-2T was 36.8 mol%. Based on 16S rRNA gene sequence analysis, strain NY-2T formed a coherent cluster with Jeotgalicoccus marinus JSM 076033T, Jeotgalicoccus halotolerans YKJ-101T and Jeotgalicoccus psychrophilus YKJ-115T. Phylogenetic analysis, DNA–DNA relatedness data, phenotypic characteristics and chemotaxonomic data indicated that strain NY-2T (=CCTCC AB 208288T =JCM 15687T) should be classified as the type strain of a novel species of the genus Jeotgalicoccus, for which the name Jeotgalicoccus huakuii sp. nov. is proposed., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.013623-0