1887

Abstract

A Gram-positive, aerobic, non-motile, rod-shaped actinomycete, designated strain DSW-2, was isolated from a seaweed sample collected around Mara Island, Jeju, Republic of Korea. Comparative 16S rRNA gene sequence analysis showed that strain DSW-2 belongs to the suborder and forms a distinct clade separated from representatives of the several families of this order. Levels of 16S rRNA gene sequence similarity between the novel strain and members of this suborder were lower than 96.4 %. The peptidoglycan type is A3 with Lys–Ser as the interpeptide bridge. Whole-cell sugars are glucose and galactose. The major menaquinone is MK-9(H). The predominant fatty acid is ai-C. The polar lipids are phosphatidylglycerol and phosphatidylinositol. The DNA G+C content was 68.3 mol%. On the basis of the chemotaxonomic markers and phylogenetic distinctiveness presented here, it is evident that the isolate represents a novel taxon within the suborder . The name gen. nov., sp. nov. is proposed, with the type strain DSW-2 (=KCTC 13436 =DSM 22126).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.014647-0
2010-07-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/7/1510.html?itemId=/content/journal/ijsem/10.1099/ijs.0.014647-0&mimeType=html&fmt=ahah

References

  1. Alonso-Vega P., Santamaría R. I., Martínez-Molina E., Trujillo M. E. 2008; Promicromonospora kroppenstedtii sp. nov., isolated from sandy soil. Int J Syst Evol Microbiol 58:1476–1481 [CrossRef]
    [Google Scholar]
  2. An D.-S., Im W.-T., Yang H.-C., Kang M. S., Kim K. K., Jin L., Kim M. K., Lee S.-T. 2005; Cellulomonas terrae sp. nov., a cellulolytic and xylanolytic bacterium isolated from soil. Int J Syst Evol Microbiol 55:1705–1709 [CrossRef]
    [Google Scholar]
  3. Bagnara C., Toci R., Gaudin C., Belaich J. P. 1985; Isolation and characterization of a cellulolytic microorganism, Cellulomonas fermentans sp. nov. Int J Syst Bacteriol 35:502–507 [CrossRef]
    [Google Scholar]
  4. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef]
    [Google Scholar]
  5. Brown J. M., Frazier R. P., Morey R. E., Steigerwalt A. G., Pellegrini G. J., Daneshvar M. I., Hollis D. G., McNeil M. M. 2005; Phenotypic and genetic characterization of clinical isolates of CDC coryneform group A-3: proposal of a new species of Cellulomonas , Cellulomonas denverensis sp. nov. J Clin Microbiol 43:1732–1737 [CrossRef]
    [Google Scholar]
  6. Brown J. M., Steigerwalt A. G., Morey R. E., Daneshvar M. I., Romero L.-J., McNeil M. M. 2006; Characterization of clinical isolates previously identified as Oerskovia turbata : proposal of Cellulosimicrobium funkei sp. nov. and emended description of the genus Cellulosimicrobium . Int J Syst Evol Microbiol 56:801–804 [CrossRef]
    [Google Scholar]
  7. Busse H.-J., Zlamala C., Buczolits S., Lubitz W., Kämpfer P., Takeuchi M. 2003; Promicromonospora vindobonensis sp. nov. and Promicromonospora aerolata sp. nov., isolated from the air in the medieval ‘Virgilkapelle’ in Vienna. Int J Syst Evol Microbiol 53:1503–1507 [CrossRef]
    [Google Scholar]
  8. Elberson M. A., Malekzadeh F., Yazdi M. T., Kameranpour N., Noori-Daloii M. R., Matte M. H., Shahamat M., Colwell R. R., Sowers K. R. 2000; Cellulomonas persica sp. nov. and Cellulomonas iranensis sp. nov., mesophilic cellulose-degrading bacteria isolated from forest soils. Int J Syst Evol Microbiol 50:993–996 [CrossRef]
    [Google Scholar]
  9. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  10. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  11. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  12. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  13. Groth I., Schumann P., Schuetze B., Augasten K., Kramer I., Stackebrandt E. 1999; Beutenbergia cavernae gen. nov., sp. nov. an L-lysine-containing actinomycete isolated from a cave. Int J Syst Bacteriol 49:1733–1740 [CrossRef]
    [Google Scholar]
  14. Groth I., Schumann P., Schütze B., Gonzalez J. M., Laiz L., Saiz-Jimenez C., Stackebrandt E. 2005; Isoptericola hypogeus sp. nov., isolated from the Roman catacomb of Domitilla. Int J Syst Evol Microbiol 55:1715–1719 [CrossRef]
    [Google Scholar]
  15. Hancock I. C. 1994; Analysis of cell wall constituents of Gram-positive bacteria. In Chemical Methods in Prokaryotic Systematics pp 63–84 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  16. Hong S. G., Lee Y. K., Yim J. H., Chun J., Lee H. K. 2008; Sanguibacter antarcticus sp. nov., isolated from Antarctic sea sand. Int J Syst Evol Microbiol 58:50–52 [CrossRef]
    [Google Scholar]
  17. Hopwood D. A., Bibb M. J., Chater K. F., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. 1985 Genetic Manipulation of Streptomyces: a Laboratory Manual Norwich: John Innes Foundation;
    [Google Scholar]
  18. Huang Y., Dai X., He L., Wang Y.-N., Wang B.-J., Liu Z., Liu S.-J. 2005; Sanguibacter marinus sp. nov., isolated from coastal sediment. Int J Syst Evol Microbiol 55:1755–1758 [CrossRef]
    [Google Scholar]
  19. Jiang Y., Wiese J., Cao Y.-R., Xu L.-H., Imhoff J. F., Jiang C.-L. 2009; Promicromonospora flava sp. nov., isolated from sediment of the Baltic Sea. Int J Syst Evol Microbiol 59:1599–1602 [CrossRef]
    [Google Scholar]
  20. Jones B. E., Grant W. D., Duckworth A. W., Schumann P., Weiss N., Stackebrandt E. 2005; Cellulomonas bogoriensis sp. nov., an alkaliphilic cellulomonad. Int J Syst Evol Microbiol 55:1711–1714 [CrossRef]
    [Google Scholar]
  21. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  22. Kalakoutskii L. V., Agre N. S., Prauser H., Evtushenko L. I. 1989; Genus Promicromonospora Krasil'nikov, Kalakoutskii and Kirillova 1961a, 107AL . In Bergey's Manual of Systematic Bacteriology vol 4 pp 2392–2395 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  23. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20) pp 173–199 Edited by Goodfellow M., Minnikin D. E. New York: Academic Press;
    [Google Scholar]
  24. Lee S. D. 2007; Labedella gwakjiensis gen. nov., sp. nov., a novel actinomycete of the family Microbacteriaceae . Int J Syst Evol Microbiol 57:2498–2502 [CrossRef]
    [Google Scholar]
  25. Lee D. W., Lee S. D. 2008; Aeromicrobium ponti sp. nov., isolated from seawater. Int J Syst Evol Microbiol 58:987–991 [CrossRef]
    [Google Scholar]
  26. Li W.-J., Zhi X.-Y., Euzéby J. P. 2008 Proposal of Yaniellaceae fam.nov., Yaniella gen. nov. and Sinobaca gen. nov.as replacements for the illegitimate prokaryotic names Yaniaceae Li et al. 2005, Yania Li et al. 2004, emend Li et al. 2005, and Sinococcus Li et al. 2006, respectively. Int J Syst Evol Microbiol 58, 525–527 [CrossRef]
  27. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  28. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. 1975; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117
    [Google Scholar]
  29. Rivas R., Trujillo M. E., Mateos P. F., Martínez-Molina E., Velázquez E. 2004; Cellulomonas xylanilytica sp. nov., a cellulolytic and xylanolytic bacterium isolated from a decayed elm tree. Int J Syst Evol Microbiol 54:533–536 [CrossRef]
    [Google Scholar]
  30. Saddler G. S., Tavecchia P., Lociuro S., Zanol M., Colombo L., Selva E. 1991; Analysis of madurose and other actinomycete whole cell sugars by gas chromatography. J Microbiol Methods 14:185–191 [CrossRef]
    [Google Scholar]
  31. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  32. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  33. Schumann P., Weiss N., Stackebrandt E. 2001; Reclassification of Cellulomonas cellulans (Stackebrandt and Keddie 1986) as Cellulosimicrobium cellulans gen. nov., comb. nov. Int J Syst Evol Microbiol 51:1007–1010 [CrossRef]
    [Google Scholar]
  34. Stackebrandt E., Schumann P. 2000; Description of Bogoriellaceae fam.nov., Dermacoccaceae fam. nov., Rarobacteraceae fam. nov. and Sanguibacteraceae fam. nov.and emendation of some families of the suborder Micrococcineae . Int J Syst Evol Microbiol 50:1279–1285 [CrossRef]
    [Google Scholar]
  35. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  36. Stackebrandt E., Schumann P., Prauser H. 2002 The family Cellulomonadaceae . In The Prokaryotes: an Evolving Electronic Resource for the Microbiological Community , release 3.0 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
    [Google Scholar]
  37. Stackebrandt E., Schumann P., Cui X.-L. 2004; Reclassification of Cellulosimicrobium variabile Bakalidou et al. , 2002 as Isoptericola variabilis gen. nov., comb. nov. Int J Syst Evol Microbiol 54:685–688 [CrossRef]
    [Google Scholar]
  38. Takahashi Y., Tanaka Y., Iwai Y., Omura S. 1987; Promicromonospora sukumoe sp. nov., a new species of the Actinomycetales . J Gen Appl Microbiol 33:507–519 [CrossRef]
    [Google Scholar]
  39. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  40. Yi H., Schumann P., Chun J. 2007; Demequina aestuarii gen. nov., sp. nov., a novel actinomycete of the suborder Micrococcineae , and reclassification of Cellulomonas fermentans Bagnara et al. 1985 as Actinotalea fermentans gen. nov., comb. nov. Int J Syst Evol Microbiol 57:151–156 [CrossRef]
    [Google Scholar]
  41. Yoon J.-H., Kang S.-J., Schumann P., Oh T.-K. 2007; Cellulosimicrobium terreum sp. nov., isolated from soil. Int J Syst Evol Microbiol 57:2493–2497 [CrossRef]
    [Google Scholar]
  42. Zhang Y.-Q., Schumann P., Li W.-J., Chen G.-Z., Tian X.-P., Stackebrandt E., Xu L.-H., Jiang C.-L. 2005; Isoptericola halotolerans sp. nov., a novel actinobacterium isolated from saline soil from Qinghai Province, north-west China. Int J Syst Evol Microbiol 55:1867–1870 [CrossRef]
    [Google Scholar]
  43. Zhi X.-Y., Li W.-J., Stackebrandt E. 2009; An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria , with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol 59:589–608 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.014647-0
Loading
/content/journal/ijsem/10.1099/ijs.0.014647-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error