1887

Abstract

A novel streptomycete, designated strain HM 35, was isolated from soil in Isfahan city, Iran. Strain HM 35 produced a branched substrate mycelium and aerial hyphae that developed into short, compact, spiral spore chains with grey rugose spores at the tips of the aerial hyphae. On some media, these spirals coalesced into dark masses of spores with age. Whole-cell hydrolysates of strain HM 35 contained -diaminopimelic acid, glucose and ribose. Phospholipids detected were phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, phosphatidylinositol mannosides, hydroxy-phosphatidylethanolamine, lyso-phosphatidylethanolamine and hydroxy-lyso-phosphatidylethanolamine. MK-9(H), MK-9(H) and MK-9(H) were the predominant menaquinones. The major fatty acids were iso- and anteiso-branched components. The chemotaxonomic characteristics of the novel isolate matched those described for members of the genus . Based on 16S rRNA gene sequence analysis, strain HM 35 showed highest similarity to NRRL 5491 (99.2 %), DSM 40563 (99.1 %), DSM 41764 (99.1 %) and DSM 41766 (99.1 %). The novel strain formed a distinct monophyletic line within the 16S rRNA gene sequence tree. The level of DNA–DNA relatedness between strain HM 35 and the type strain of was 72.7 %. Strain HM 35 showed the typical morphology found among members of the / group but could be clearly differentiated from closely related species based on other phenotypic markers. Phenotypic and genotypic data thus indicate that strain HM 35 represents a novel species of the genus , for which the name is proposed. The type strain is HM 35 (=DSM 41954=CCUG 57623).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.015339-0
2010-07-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/7/1504.html?itemId=/content/journal/ijsem/10.1099/ijs.0.015339-0&mimeType=html&fmt=ahah

References

  1. Berdy J. 1995; Are actinomycetes exhausted as a source of secondary metabolites?. Biotechnologia 7:813–34
    [Google Scholar]
  2. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef]
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  4. Euzéby J. P. 2009 List of Prokaryotic names with Standing in Nomenclature ; http://www.bacterio.cict.fr
    [Google Scholar]
  5. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  6. Fiedler H.-P., Bruntner C., Bull A. T., Ward A. C., Goodfellow M., Potterat O., Puder C., Mihm G. 2005; Marine actinomycetes as a source of novel secondary metabolites. Antonie van Leeuwenhoek 87:37–42 [CrossRef]
    [Google Scholar]
  7. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  8. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome C sequences is of general applicability. Science 155:279–284 [CrossRef]
    [Google Scholar]
  9. Hayakawa M., Kajiura T., Nonomura H. 1991; New methods for the highly selective isolation of Streptosporangium and Dactylosporangium from soil. J Ferment Bioeng 72:327–333 [CrossRef]
    [Google Scholar]
  10. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  11. Kämpfer P. 2006; The family Streptomycetaceae Part I: Taxonomy. In The Prokaryotes vol 3 pp 538–604 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
    [Google Scholar]
  12. Kawato N., Shinobu R. 1959; Streptomyces herbaricolor sp. nov., supplement: a single technique for microscopical observation. Mem Osaka Univ Lib Arts Educ B 8:114–119
    [Google Scholar]
  13. Kelly K. L. 1964 Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
    [Google Scholar]
  14. Kim S. B., Goodfellow M. 2002; S treptomyces avermitilis sp. , nov., nom. rev. a taxonomic home for the avermectin-producing streptomycetes. Int J Syst Evol Microbiol 522011–2014 [CrossRef]
    [Google Scholar]
  15. Kroppenstedt R. M. 1982; Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5:2359–2367 [CrossRef]
    [Google Scholar]
  16. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics pp 173–179 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  17. Kroppenstedt R. M. 1992; The genus Nocardiopsis . In The Prokaryotes pp 1139–1156 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer;
    [Google Scholar]
  18. Kroppenstedt R. M., Evtushenko L. I. 2006; The family Nocardiopsaceae . In The Prokaryotes: a Handbook on the Biology of Bacteria . , 3rd edn. vol 3 pp 754–795 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
  19. Labeda D. P. 1998; DNA relatedness among the Streptomyces fulvissimus and Streptomyces griseoviridis phenotypic cluster groups. Int J Syst Bacteriol 48:829–832 [CrossRef]
    [Google Scholar]
  20. Lanoot B., Vancanneyt M., Dawyndt P., Cnockaert M., Zhang Z., Huang Y., Liu Z., Swings J. 2004; BOX-PCR fingerprinting as a powerful tool to reveal synonymous names in the genus Streptomyces . Emended descriptions of the species Streptomyces cinereorectus , S. fradiae , S. tricolor , S. colombiensis , S. filamentosus , S. vinaceus and S. phaeopurpureus . Syst Appl Microbiol 27:84–92 [CrossRef]
    [Google Scholar]
  21. Lechevalier M. P., Lechevalier H. A. 1980; The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy, Special Publication 6 pp 227–291 Arlington, VA: Society for Industrial Microbiology;
    [Google Scholar]
  22. Lechevalier M. P., De Bièvre C., Lechevalier H. A. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260 [CrossRef]
    [Google Scholar]
  23. Mesbah M., Premachandran U., Whitman W. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  24. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  25. Nonomura H., Ohara Y. 1971; Distribution of actinomycetes in soil. J Ferm Technol 49:904–912
    [Google Scholar]
  26. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [CrossRef]
    [Google Scholar]
  27. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  28. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  29. Sembiring L., Ward A. C., Goodfellow M. 2000; Selective isolation and characterisation of members of the Streptomyces violaceusniger clade associated with the roots of Paraserianthes falcataria . Antonie van Leeuwenhoek 78:353–366 [CrossRef]
    [Google Scholar]
  30. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  31. Shirling E. B., Gottlieb D. 1972; Comparative description of type strains of Streptomyces . Int J Syst Bacteriol 22:265–394 [CrossRef]
    [Google Scholar]
  32. Stackebrandt E., Ebers J. 2006; Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155
    [Google Scholar]
  33. Staneck J. L., Roberts G. D. 1974; Simplified approach to the identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  34. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  35. Waksman S. A., Henrici A. T. 1943; The nomenclature and classification of the actinomycetes. J Bacteriol 46:337–341
    [Google Scholar]
  36. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  37. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983; Numerical classification of Streptomyces and related taxa. J Gen Microbiol 129:1743–1813
    [Google Scholar]
  38. Williams S. T., Goodfellow M., Alderson G. 1989; Genus Streptomyces Waksman and Henrici 1943, 339AL. In Bergey's Manual of Systematic Bacteriology vol. 4 pp 2452–2492 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.015339-0
Loading
/content/journal/ijsem/10.1099/ijs.0.015339-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error