1887

Abstract

Strain FhSp1, isolated from human blood in Spain in 2003, was studied for its taxonomic allocation. By 16S rRNA and gene sequencing, the strain was shown to belong to the genus . In the 16S rRNA gene sequence, sp. FhSp1 shared similarity of more than 99 % with strains of subspecies and U112, 98 % with GM2212 and 98.4 % with ATCC 25015. In the gene sequence, sp. FhSp1 exhibited 91.6–91.7 % similarity to strains of subspecies, 91.2 % to U112 and 84 % to ATCC 25017. The genus affiliation was supported by a quinone system typical of (Q-8 as the major component), a complex polar lipid profile similar to that of with the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and an unknown aminophospholipid (APL4) and a fatty acid profile consisting mainly of C (17.2 %), C (11.2 %), C (13.1 %), C 3-OH (14.2 %) and C 9 (7.1 %). DNA–DNA hybridization, which showed unambiguously that FhSp1 represents a novel species, and the results of biochemical tests allowed genotypic and phenotypic differentiation of the isolate from all hitherto-described species. A multiplex PCR developed in the course of this study discriminated FhSp1 from representatives of all other species and subspecies, clades A.I and A.II of subsp. and subsp. biovar japonica and also between these representatives of the genus. Therefore, we propose the name sp. nov., with the type strain FhSp1 (=FnSp1 =FSC454 =F62 =DSM 22475 =CCUG 58020). Furthermore, we formally propose the transfer of the species to the species as subsp. comb. nov. (type strain ATCC 15482 =CCUG 33449 =CIP 56.12). We also present an emended description of the genus .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.015941-0
2010-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/8/1887.html?itemId=/content/journal/ijsem/10.1099/ijs.0.015941-0&mimeType=html&fmt=ahah

References

  1. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J. 1996; Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47:39–52 [CrossRef]
    [Google Scholar]
  2. Broekhuijsen M., Larsson P., Johansson A., Byström M., Eriksson U., Larsson E., Prior R. G., Sjöstedt A., Titball R. W., Forsman M. 2003 Genome-wide DNA microarray analysis of Francisella tularensis strains demonstrates extensive genetic conservation within the species but identifies regions that are unique to the highly virulent F.tularensis subsp. tularensis . J Clin Microbiol 41, 2924–2931 [CrossRef]
  3. Escudero R., Elía M., Sáez-Nieto J. A., Herrera L., Galán J. A., Ruiz M., Menéndez V., Royo G., Anda P. 2003 Preliminary characterization of a human isolate of Francisella novicida .In Abstracts of the 4th International Conference on Tularemia , 15–18 September 2003, Bath, UK, p. 30
  4. Escudero R., Toledo A., Gil H., Kovácsová K., Rodríguez-Vargas M., Jado I., García-Amil C., Lobo B., Bhide M. 2008; Molecular method for discrimination between Francisella tularensis and Francisella -like endosymbionts. J Clin Microbiol 46:3139–3143 [CrossRef]
    [Google Scholar]
  5. Escudero R., Elía M., Sáez-Nieto J. A., Menéndez V., Toledo A., Royo G., Rodríguez-Vargas M., Whipp M. J., Gil H. other authors 2010; A possible novel Francisella genomic species isolated from blood and urine of a patient with severe illness. Clin Microbiol Infect 16:1026–1030 [CrossRef]
    [Google Scholar]
  6. Forsman M., Sandstrom G., Sjöstedt A. 1994; Analysis of 16S ribosomal DNA sequences of Francisella strains and utilization for determination of the phylogeny of the genus and for identification of strains by PCR. Int J Syst Bacteriol 44:38–46 [CrossRef]
    [Google Scholar]
  7. Gao B., Mohan R., Gupta R. S. 2009; Phylogenomics and protein signatures elucidating the evolutionary relationships among the Gammaproteobacteria . Int J Syst Evol Microbiol 59:234–247 [CrossRef]
    [Google Scholar]
  8. Hollis D. G., Weaver R. E., Steigerwalt A. G., Wenger J. D., Moss C. W., Brenner D. J. 1989; Francisella philomiragia comb. nov. (formerly Yersinia philomiragia ) and Francisella tularensis biogroup novicida (formerly Francisella novicida ) associated with human disease. J Clin Microbiol 27:1601–1608
    [Google Scholar]
  9. Jantzen E., Berdal B. P., Omland T. 1979; Cellular fatty acid composition of Francisella tularensis . J Clin Microbiol 10:928–930
    [Google Scholar]
  10. Johansson A., Ibrahim A., Göransson I., Eriksson U., Gurycova D., Clarridge J. E., Sjöstedt A. 2000; Evaluation of PCR-based methods for discrimination of Francisella species and subspecies and development of a specific PCR that distinguishes the two major subspecies of Francisella tularensis . J Clin Microbiol 38:4180–4185
    [Google Scholar]
  11. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  12. Kugeler K. J., Mead P. S., McGowan K. L., Burnham J. M., Hogarty M. D., Ruchelli E., Pollard K., Husband B., Conley C. other authors 2008; Isolation and characterization of a novel Francisella sp. from human cerebrospinal fluid and blood. J Clin Microbiol 46:2428–2431 [CrossRef]
    [Google Scholar]
  13. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp 115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  14. Larson C. L., Wicht W., Jellison W. L. 1955; A new organism resembling P. tularensis isolated from water. Public Health Rep 70:253–258 [CrossRef]
    [Google Scholar]
  15. Larsson P., Svensson K., Karlsson L., Guala D., Granberg M., Forsman M., Johansson A. 2007; Canonical insertion-deletion markers for rapid DNA typing of Francisella tularensis . Emerg Infect Dis 13:1725–1732 [CrossRef]
    [Google Scholar]
  16. Lau K. W. K., Ren J., Fung M.-C., Woo P. C. Y., Yuen K.-Y., Chan K. K. M., Qian P.-Y., Wong P.-K., Wu M. 2007; Fangia hongkongensis gen. nov., sp. nov., a novel gammaproteobacterium of the order Thiotrichales isolated from coastal seawater of Hong Kong. Int J Syst Evol Microbiol 57:2665–2669 [CrossRef]
    [Google Scholar]
  17. Leelaporn A., Yongyod S., Limsrivanichakorn S., Yungyuen T., Kiratisin P. 2008; Francisella novicida bacteremia, Thailand. Emerg Infect Dis 14:1935–1937 [CrossRef]
    [Google Scholar]
  18. McCoy G. W., Chapin C. W. 1912; Further observations on a plague-like disease of rodents with a preliminary note on the causative agent, Bacterium tularense . J Infect Dis 10:61–72 [CrossRef]
    [Google Scholar]
  19. Mikalsen J., Olsen A. B., Tengs T., Colquhoun D. J. 2007 Francisella philomiragia subsp. noatunensis subsp. nov., isolated from farmed Atlantic cod ( Gadus morhua L.). Int J Syst Evol Microbiol 57, 1960–1965. [CrossRef]
  20. Molins-Schneekloth C. R., Belisle J. T., Petersen J. M. 2007; Genomic markers for differentiation of Francisella tularensis subsp. tularensis A.I and A.II. Appl Environ Microbiol 74:336–341
    [Google Scholar]
  21. Muyzer G., de Waal E. C., Uitterlinden A. G. 1993; Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59:695–700
    [Google Scholar]
  22. Nübel U, Reissbrodt R., Weller A., Grunow R., Porsch-Özcürümez M, Tomaso H., Hofer E., Splettstoesser W., Finke E.-J. other authors 2006; Population structure of Francisella tularensis . J Bacteriol 188:5319–5324 [CrossRef]
    [Google Scholar]
  23. Olsufiev N. G., Emelyanova O. S., Dunaeva T. N. 1959; Comparative studies of strains of B. tularense in the Old and New World and their taxonomy. J Hyg Epidemiol Microbiol Immunol 3:138–149
    [Google Scholar]
  24. Olsufjev N. G., Meshcheryakova I. S. 1983; Subspecific taxonomy of Francisella tularensis McCoy and Chapin 1912. Int J Syst Bacteriol 33:872–874 [CrossRef]
    [Google Scholar]
  25. Ottem K. F., Nylund A., Karlsbakk E., Friis-Møller A., Krossoy B., Knappskog D. 2007; New species in the genus Francisella . (Gammaproteobacteria; Francisellaceae); Francisella piscicida sp. nov. isolated from cod ( Gadus morhua ). Arch Microbiol 188, 547–550 [CrossRef]
  26. Ottem K. F., Nylund A., Karlsbakk E., Friis-Møller A, Kamaishi T. 2009; Elevation of Francisella philomiragia subsp. noatunensis Mikalsen et al. (2007) to Francisella noatunensis comb.nov. [syn. Francisella piscicida Ottem et al. (2008) syn. nov.] and characterization of Francisella noatunensis subsp. orientalis subsp. nov., two important fish pathogens. J Appl Microbiol 106: 1231–1243 [CrossRef]
    [Google Scholar]
  27. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85:2444–2448 [CrossRef]
    [Google Scholar]
  28. Pitcher D. G., Saunders N. A., Owen R. J. 1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151–156 [CrossRef]
    [Google Scholar]
  29. Richards G. M. 1974; Modifications of the diphenylamine reaction giving increased sensitivity and simplicity in the estimation of DNA. Anal Biochem 57:369–376 [CrossRef]
    [Google Scholar]
  30. Scholz H. C., Tomaso H., Al Dahouk S., Witte A., Schloter M., Kämpfer P., Falsen E., Neubauer H. 2006; Genotyping of Ochrobactrum anthropi by recA -based comparative sequence, PCR-RFLP, and 16S rRNA gene analysis. FEMS Microbiol Lett 257:7–16 [CrossRef]
    [Google Scholar]
  31. Sjöstedt A. B. 2005; Genus I. Francisella Dorofe'ev 1947, 176AL. In Bergey's Manual of Systematic Bacteriology . , 2nd edn. vol. 2, part B pp 200–210 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
  32. Stolz A., Busse H.-J., Kämpfer P. 2007; Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 57:572–576 [CrossRef]
    [Google Scholar]
  33. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  34. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  35. Tindall B. J. 1990a; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [CrossRef]
    [Google Scholar]
  36. Tindall B. J. 1990b; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [CrossRef]
    [Google Scholar]
  37. Wieser M., Busse H.-J. 2000; Rapid identification of Staphylococcus epidermidis . Int J Syst Evol Microbiol 50:1087–1093 [CrossRef]
    [Google Scholar]
  38. Ziemke F., Höfle M. G., Lalucat J., Rosselló-Mora R. 1998; Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48:179–186 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.015941-0
Loading
/content/journal/ijsem/10.1099/ijs.0.015941-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error