RT Journal Article SR Electronic(1) A1 Supré, Karlien A1 De Vliegher, Sarne A1 Cleenwerck, Ilse A1 Engelbeen, Katrien A1 Van Trappen, Stefanie A1 Piepers, Sofie A1 Sampimon, Otlis C. A1 Zadoks, Ruth N. A1 De Vos, Paul A1 Haesebrouck, FreddyYR 2010 T1 Staphylococcus devriesei sp. nov., isolated from teat apices and milk of dairy cows JF International Journal of Systematic and Evolutionary Microbiology, VO 60 IS 12 SP 2739 OP 2744 DO https://doi.org/10.1099/ijs.0.015982-0 PB Microbiology Society, SN 1466-5034, AB Ten non-motile, Gram-stain-positive, coagulase-negative staphylococci were isolated from bovine milk and teat apices. All isolates were catalase-positive, with anteiso-C15 : 0, iso-C15 : 0, anteiso-C17 : 0, iso-C17 : 0 and C18 : 0 as predominant fatty acids and diphosphatidylglycerol and phosphatidylglycerol as major polar lipids. The results of sequence analysis of the 16S rRNA gene and four housekeeping genes (rpoB, hsp60, tuf and dnaJ) in combination with tRNA-intergenic spacer length analysis showed that the isolates form a separate branch within the genus Staphylococcus. Based on 16S rRNA gene sequencing, the phylogenetically most closely related species are Staphylococcus haemolyticus, S. hominis and S. lugdunensis, with >98.7 % sequence similarity. The DNA G+C content varies from 33.3 to 33.7 mol%, and DNA–DNA hybridization with the nearest neighbours, based on 16S rRNA gene sequences, confirmed that the isolates represent a novel Staphylococcus species. All isolates induced a small zone of complete haemolysis on Columbia agar with 5 % sheep blood and exhibited a homogeneous biochemical fingerprint that is discriminative from the phylogenetically most closely related species. Based on these results, it is proposed to classify the ten isolates as Staphylococcus devriesei sp. nov., with strain KS-SP 60T (=LMG 25332T =CCUG 58238T) as the type strain., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.015982-0