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Volume 60, Issue 8

sp. nov., a marine bacterium isolated from the starfish Free

Abstract

A marine bacterial strain, KMD 002, was isolated from an Amur starfish, , collected in the East Sea of Korea. Strain KMD 002 was a Gram-negative, beige-pigmented, rod-shaped bacterium. The strain was capable of growth at relatively low temperatures (4–25 °C) and over a broad pH range (pH 4.0–10.0). The major fatty acids were C 7 and/or iso-C 2-OH and C and the predominant isoprenoid quinone was Q-8. The DNA G+C content of strain KMD 002 was 40.3 mol%. Phylogenetic analysis using 16S rRNA gene sequences revealed that strain KMD 002 belonged to the genus . However, various phenotypic properties as well as low 16S rRNA gene sequence similarities to members of the genus (94.1–96.7 %) suggested that strain KMD 002 is a representative of a novel species, for which the name sp. nov. is proposed. The type strain is KMD 002 (=KCCM 90077 =JCM 15608).

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2010-08-01
2024-04-24
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [CrossRef]
     [Google Scholar]
  2. Bligh E. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917 [CrossRef]
     [Google Scholar]
  3. Bowman J. P., Gosink J. J., McCammon S. A., Lewis T. E., Nichols D. S., Nichols P. D., Skerratt J. H., Staley J. T., McMeekin T. A. 1998 Colwellia demingiae sp. nov., Colwellia hornerae sp. nov., Colwellia rossensis sp. nov. and Colwellia psychrotropica sp. nov.: psychrophilic Antarctic species with the ability to synthesize docosahexaenoic acid (22 : 6 ω 3). Int J Syst Bacteriol 48, 1171–1180 [CrossRef]
  4. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef]
     [Google Scholar]
  5. Choi E. J., Kwon H. C., Sohn Y. C., Yang H. O. 2010; Kistimonas asteriae gen. nov., sp. nov. a gammaproteobacterium isolated from Asterias amurensis . Int J Syst Evol Microbiol 60:938–943 [CrossRef]
     [Google Scholar]
  6. Collins M. D., Jones D. J. 1981; A note on the separation of natural mixtures of bacterial ubiquinones using reverse-phase partition thin-layer chromatography and high performance liquid chromatography. J Appl Bacteriol 51:129–134 [CrossRef]
     [Google Scholar]
  7. D'Aoust J. Y., Kushner D. J. 1972; Vibrio psychroerythrus sp. n.: classification of the psychrophilic marine bacterium, NRC 1004. J Bacteriol 111:340–342
     [Google Scholar]
  8. Deming J. W., Somers L. K., Straube W. L., Swartz D. G., MacDonell M. T. 1988; Isolation of an obligately barophilic bacterium and description of a new genus, Colwellia gen. nov. Syst Appl Microbiol 10:152–160 [CrossRef]
     [Google Scholar]
  9. Hsu S. C., Lockwood J. L. 1975; Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Appl Microbiol 29:422–426
     [Google Scholar]
  10. Ivanova E. P., Flavier S., Christen R. 2004; Phylogenetic relationships among marine Alteromonas -like proteobacteria: emended description of the family Alteromonadaceae and proposal of Pseudoalteromonadaceae fam.nov., Colwelliaceae fam. nov., Shewanellaceae fam. nov., Moritellaceae fam. nov., Ferrimonadaceae fam. nov., Idiomarinaceae fam. nov. and Psychromonadaceae fam. nov. Int J Syst Evol Microbiol 54:1773–1788 [CrossRef]
     [Google Scholar]
  11. Jung S. Y., Oh T. K., Yoon J. H. 2006; Colwellia aestuarii sp. nov., isolated from a tidal flat sediment in Korea. Int J Syst Evol Microbiol 56:33–37 [CrossRef]
     [Google Scholar]
  12. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
     [Google Scholar]
  13. Martin K., Schumann P., Rainey F. A., Schuetze B., Groth I. 1997; Janibacter limosus gen. nov., sp. nov. a new actinomycete with meso -diaminopimelic acid in the cell wall. Int J Syst Bacteriol 47:529–534 [CrossRef]
     [Google Scholar]
  14. Nogi Y., Kato C. 1999; Taxonomic studies of extremely barophilic bacteria isolated from the Mariana Trench and description of Moritella yayanosii sp. nov., a new barophilic bacterial isolate. Extremophiles 3:71–77 [CrossRef]
     [Google Scholar]
  15. Nogi Y., Hosoya S., Kato C., Horikoshi K. 2004; Colwellia piezophila sp. nov., a novel piezophilic species from deep-sea sediments of the Japan Trench. Int J Syst Evol Microbiol 54:1627–1631 [CrossRef]
     [Google Scholar]
  16. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
     [Google Scholar]
  17. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology . pp 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  18. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
     [Google Scholar]
  19. Süßmuth R., Eberspächer J., Haag R., Springer W. 1987 Biochemisch-mikrobiologisches Praktikum Stuttgart: Thieme (in German;
     [Google Scholar]
  20. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
     [Google Scholar]
  21. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
     [Google Scholar]
  22. Yi H., Bae K. S., Chun J. 2004; Thalassomonas ganghwensis sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54:377–380 [CrossRef]
     [Google Scholar]
  23. Yumoto I., Kawasaki K., Iwata H., Matsuyama H., Okuyama H. 1998; Assignment of Vibrio sp. strain ABE-1 to Colwellia maris sp. nov., a new psychrophilic bacterium. Int J Syst Bacteriol 48:1357–1362 [CrossRef]
     [Google Scholar]
  24. Zhang D.-C., Yu Y., Xin Y.-H., Liu H.-C., Zhou P.-J., Zhou Y.-G. 2008; Colwellia polaris sp. nov., a psychrotolerant bacterium isolated from Arctic sea ice. Int J Syst Evol Microbiol 58:1931–1934 [CrossRef]
     [Google Scholar]
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Colwellia asteriadis sp. nov., a marine bacterium isolated from the starfish Asterias amurensis
Int J Syst Evol Microbiol 60, 1952 (2010); https://doi.org/10.1099/ijs.0.016055-0
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