RT Journal Article SR Electronic(1) A1 Steinsbu, Bjørn O. A1 Thorseth, Ingunn H. A1 Nakagawa, Satoshi A1 Inagaki, Fumio A1 Lever, Mark A. A1 Engelen, Bert A1 Øvreås, Lise A1 Pedersen, Rolf B.YR 2010 T1 Archaeoglobus sulfaticallidus sp. nov., a thermophilic and facultatively lithoautotrophic sulfate-reducer isolated from black rust exposed to hot ridge flank crustal fluids JF International Journal of Systematic and Evolutionary Microbiology, VO 60 IS 12 SP 2745 OP 2752 DO https://doi.org/10.1099/ijs.0.016105-0 PB Microbiology Society, SN 1466-5034, AB A novel thermophilic and lithoautotrophic sulfate-reducing archaeon was isolated from black rust formed on the steel surface of a borehole observatory (CORK 1026B) retrieved during IODP Expedition 301 on the eastern flank of Juan de Fuca Ridge, eastern Pacific Ocean. Cells of the strain were lobe-shaped or triangular. The optimum temperature, pH and NaCl concentration for growth were 75 °C, pH 7 and 2 % (w/v), respectively. The isolate was strictly anaerobic, growing lithoautotrophically on H2 and CO2 using sulfate, sulfite or thiosulfate as electron acceptors. Lactate and pyruvate could serve as alternative energy and carbon sources. The G+C content of the genomic DNA was 42 mol%. Phylogenetic analyses of the 16S rRNA gene indicated that the isolate was closely related to members of the family Archaeoglobaceae, with sequence similarities of 90.3–94.4 %. Physiological and molecular properties showed that the isolate represents a novel species of the genus Archaeoglobus. The name Archaeoglobus sulfaticallidus sp. nov. is proposed; the type strain is PM70-1T (=DSM 19444T=JCM 14716T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.016105-0