1887

Abstract

A Gram-staining-positive, endospore-forming, motile, rod-shaped bacterium, strain AD-1, was isolated from a tidal flat sediment of the coast of Hwangdo on the Yellow Sea, Korea. Strain AD-1 grew optimally at pH 7.0–7.5 and 40 °C and in the presence of 5–10 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain AD-1 was most closely related to G-19.1 (98.0 % sequence similarity) and HS286 (97.8 %). The cell-wall peptidoglycan was based on -diaminopimelic acid and MK-7 was the predominant menaquinone. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and two unidentified lipids. The major fatty acids (>10 % of total fatty acids) were iso-C, iso-C and anteiso-C. The DNA G+C content of strain AD-1 was 45.2 mol%. It appears reasonable to classify strain AD-1 as a member of the genus . There were differences in fatty acid profiles and phenotypic and genetic characteristics between strain AD-1 and the type strains of the two species. On the basis of the data presented, strain AD-1 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is AD-1 (=KCTC 13254 =CCUG 56607).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.016204-0
2010-09-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/9/2108.html?itemId=/content/journal/ijsem/10.1099/ijs.0.016204-0&mimeType=html&fmt=ahah

References

  1. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov. a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 511997–2006 [CrossRef]
    [Google Scholar]
  2. Choi D. H., Yi H., Chun J., Cho B. C. 2006; Jannaschia seosinensis sp. nov., isolated from hypersaline water of a solar saltern in Korea. Int J Syst Evol Microbiol 56:45–49 [CrossRef]
    [Google Scholar]
  3. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  5. Farrow J. A. E., Ash C., Wallbanks S., Collins M. D. 1992; Phylogenetic analysis of the genera Planococcus , Marinococcus and Sporosarcina and their relationships to members of the genus Bacillus . FEMS Microbiol Lett 93:167–172 [CrossRef]
    [Google Scholar]
  6. García M. T., Gallego V., Ventosa A., Mellado E. 2005; Thalassobacillus devorans gen. nov., sp. nov. a moderately halophilic, phenol-degrading, Gram-positive bacterium. Int J Syst Evol Microbiol 55:1789–1795 [CrossRef]
    [Google Scholar]
  7. Hanada S., Liu W. T., Shintani T., Kamagata Y., Nakamura K. 2002; Tetrasphaera elongata sp. nov., a polyphosphate-accumulating bacterium isolated from activated sludge. Int J Syst Evol Microbiol 52:883–887 [CrossRef]
    [Google Scholar]
  8. Hua N.-P., Kanekiyo A., Fujikura K., Yasuda H., Naganuma T. 2007; Halobacillus profundi sp. nov. and Halobacillus kuroshimensis sp. nov., moderately halophilic bacteria isolated from a deep-sea methane cold seep. Int J Syst Evol Microbiol 57:1243–1249 [CrossRef]
    [Google Scholar]
  9. Jung Y.-T., Kim B.-H., Oh T.-K., Yoon J.-H. 2010; Pseudoruegeria lutimaris sp. nov., isolated from a tidal flat sediment, and emended description of the genus Pseudoruegeria . Int J Syst Evol Microbiol 60:1177–1181 [CrossRef]
    [Google Scholar]
  10. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  11. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  12. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  13. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  14. Murray R. G. E., Doetsch R. N., Robinow C. F. 1994; Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology pp 21–41 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  15. Nam J.-H., Bae W., Lee D.-H. 2008; Oceanobacillus caeni sp. nov., isolated from a Bacillus -dominated wastewater treatment system in Korea. Int J Syst Evol Microbiol 58:1109–1113 [CrossRef]
    [Google Scholar]
  16. Sanchez-Porro C., Amoozegar M. A., Rohban R., Hajighasemi M., Ventosa A. 2009; Thalassobacillus cyri sp. nov., a moderately halophilic Gram-positive bacterium from a hypersaline lake. Int J Syst Evol Microbiol 59:2565–2570 [CrossRef]
    [Google Scholar]
  17. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  18. Sehgal S. N., Gibbons N. E. 1960; Effect of some metal ions on the growth of Halobacterium cutirubrum . Can J Microbiol 6:165–169 [CrossRef]
    [Google Scholar]
  19. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology. pp 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  20. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  21. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  22. Ventosa A., Quesada E., Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. 1982; Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128:1959–1968
    [Google Scholar]
  23. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  24. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  25. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
    [Google Scholar]
  26. Yoon J.-H., Kang S.-S., Lee K.-C., Kho Y. H., Kang K. H., Park Y.-H. 2001 Planomicrobium koreense gen. nov., sp. nov., a bacterium isolated from the Korean traditional fermented seafood jeotgal, and transfer of Planococcus okeanokoites (Nakagawa et al . 1996) and Planococcus mcmeekinii (Junge et al . 1998) to the genus Planomicrobium . Int J Syst Evol Microbiol 51, 1511–1520.
  27. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H. 2003; Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53:53–57 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.016204-0
Loading
/content/journal/ijsem/10.1099/ijs.0.016204-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error