Oceanobacillus neutriphilus sp. nov., isolated from activated sludge in a bioreactor Yang, Jun-Yi and Huo, Ying-Yi and Xu, Xue-Wei and Meng, Fan-Xu and Wu, Min and Wang, Chun-Sheng,, 60, 2409-2414 (2010), doi = https://doi.org/10.1099/ijs.0.016295-0, publicationName = Microbiology Society, issn = 1466-5026, abstract= A Gram-stain-positive, neutrophilic, rod-shaped bacterium, strain A1gT, was isolated from activated sludge of a bioreactor and was subjected to a polyphasic taxonomic characterization. The isolate grew in the presence of 0–17.0 % (w/v) NaCl and at pH 6.0–9.0; optimum growth was observed in the presence of 3.0–5.0 % (w/v) NaCl and at pH 7.0. Strain A1gT was motile, formed cream-coloured colonies, was catalase- and oxidase-positive and was able to hydrolyse aesculin, Tween 40 and Tween 60. Chemotaxonomic analysis revealed menaquinone-7 as the predominant respiratory quinone and anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and iso-C15 : 0 as major fatty acids. The genomic DNA G+C content of strain A1gT was 36.3 mol%. Comparative 16S rRNA gene sequence analysis revealed that the new isolate belonged to the genus Oceanobacillus and exhibited closest phylogenetic affinity to the type strains of Oceanobacillus oncorhynchi subsp. incaldanensis (97.9 % similarity) and O. oncorhynchi subsp. oncorhynchi (97.5 %), but less than 97 % sequence similarity with respect to the type strains of other recognized Oceanobacillus species. Levels of DNA–DNA relatedness between strain A1gT and reference strains O. oncorhynchi subsp. incaldanensis DSM 16557T, O. oncorhynchi subsp. oncorhynchi JCM 12661T and Oceanobacillus iheyensis DSM 14371T were 29, 45 and 38 %, respectively. On the basis of phenotypic and genotypic data, strain A1gT is considered to represent a novel species of the genus Oceanobacillus, for which the name Oceanobacillus neutriphilus sp. nov. is proposed. The type strain is A1gT (=CGMCC 1.7693T =JCM 15776T)., language=, type=