1887

Abstract

A spore-forming anaerobic bacterium, designated strain P6, was isolated from the sludge of an up-flow anaerobic sludge blanket reactor treating brewery wastewater. Cells were Gram-positive, oval and 0.6–0.9 μm by 1.2–1.8 μm in size. Growth was observed at 20–42 °C and at pH 5.0–7.5. It fermented several hexoses, polysaccharides and alcohols. Sucrose and aesculin could also be fermented. The main end products of fermentation from glucose were acetate, lactate and fumarate; trace CO and H were also produced. The DNA G+C content of strain P6 was 55.6 mol%. The major cellular fatty acids were iso-C, anteiso-C and iso-C 3-OH. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain P6 represented a novel phyletic sublineage in clostridial cluster III, and showed <91 % similarity to the type strains of recognized species in this cluster. Phenotypically, the new isolate was distinguished from its phylogenetic relatives (e.g. , , and ) by producing acid from glucose and its inability to degrade cellulose. On the basis of evidence from this polyphasic study, strain P6 is considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is P6 (=JCM 14006 =AS 1.5064).

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2010-12-01
2024-03-29
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References

  1. Chen S., Dong X. 2004; Acetanaerobacterium elongatum gen. nov., sp. nov., from paper mill wastewater. Int J Syst Evol Microbiol 54:2257–2262 [CrossRef]
    [Google Scholar]
  2. Collins M. D., Lawson P. A., Willems A., Cordoba J. J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J. A. E. 1994; The phylogeny of the genus Clostridium : proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44:812–826 [CrossRef]
    [Google Scholar]
  3. Falsen E., Collins M. D., Welinder-Olsson C., Song Y., Finegold S. M., Lawson P. A. 2005; Fastidiosipila sanguinis gen. nov., sp. nov., a new Gram-positive, coccus-shaped organism from human blood. Int J Syst Evol Microbiol 55:853–858 [CrossRef]
    [Google Scholar]
  4. Holdeman L. V., Cato E. P., Moore W. E. C. 1977 Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University;
    [Google Scholar]
  5. Hungate R. E. 1969; A roll-tube method for cultivation of strict anaerobes. Methods Microbiol 3B:117–132
    [Google Scholar]
  6. Kaneda T. 1991; Iso- and anteiso-fatty acids in bacteria: biosynthesis, function, and taxonomic significance. Microbiol Rev 55:288–302
    [Google Scholar]
  7. Kato S., Haruta S., Cui Z. J., Ishii M., Yokota A., Igarashi Y. 2004; Clostridium straminisolvens sp. nov., a moderately thermophilic, aerotolerant and cellulolytic bacterium isolated from a cellulose-degrading bacterial community. Int J Syst Evol Microbiol 54:2043–2047 [CrossRef]
    [Google Scholar]
  8. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; mega2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  9. Liu W. T., Chan O. C., Fang H. H. 2002; Characterization of microbial community in granular sludge treating brewery wastewater. Water Res 36:1767–1775 [CrossRef]
    [Google Scholar]
  10. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  11. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  12. McBee R. H. 1954; The characteristics of Clostridium thermocellum . J Bacteriol 67:505–506
    [Google Scholar]
  13. Miller L. T. 1982; Single derivatization method for routine analysis of bacterial whole-cell fatty acid-methyl esters, including hydroxy acids. J Clin Microbiol 16:584–586
    [Google Scholar]
  14. Patel G. B., Khan A. W., Agnew B. J., Colvin J. R. 1980; Isolation and characterization of an anaerobic, cellulolytic microorganism, Acetivibrio cellulolyticus gen. nov., sp. nov. . Int J Syst Bacteriol 30:179–185 [CrossRef]
    [Google Scholar]
  15. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  16. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  17. Yang J. C., Chynoweth D. P., Williams D. S., Li A. 1990; Clostridium aldrichii sp. nov., a cellulolytic mesophile inhabiting a wood-fermenting anaerobic digester. Int J Syst Bacteriol 40:268–272 [CrossRef]
    [Google Scholar]
  18. Zehnder A. J. B. 1978; Ecology of methane formation. In Water Pollution Microbiology pp 349–376 Edited by Mitchell R. New York: Wiley;
    [Google Scholar]
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