1887

Abstract

-like isolates, strains W03-356, W03-357 and W03-359, from three independent water samples from the river Elbe, Germany, were analysed by using a polyphasic approach. Morphological and biochemical characterization revealed that they were Gram-negative, aerobic, non-spore-forming bacilli with a cut glass colony appearance that grew only on -cysteine-supplemented buffered charcoal yeast extract agar. Phylogenetic analysis based on sequence comparisons of the 16S rRNA, macrophage infectivity potentiator (), gyrase subunit A (), ribosomal polymerase B () and RNase P () genes confirmed that the three isolates were distinct from recognized species of the genus . Phenotypic characterization of strain W03-356 based on fatty acid profiles confirmed that it was closely related to ATCC 35304 and ATCC 33152, but distinct from other species of the genus . Serotyping of the isolates showed that they were distinct from all recognized species of the genus . Strains W03-356, W03-357 and W03-359 are thus considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is W03-356 (=DSM 19488=NCTC 13409).

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2010-11-01
2024-04-19
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References

  1. Adeleke A. A., Fields B. S., Benson R. F., Daneshvar M. I., Pruckler J. M., Ratcliff R. M., Harrison T. G., Weyant R. S., Birtles R. J. other authors 2001; Legionella drozanskii sp. nov., Legionella rowbothamii sp. nov. and Legionella fallonii sp. nov.: three unusual new Legionella species. Int J Syst Evol Microbiol 51:1151–1160 [CrossRef]
    [Google Scholar]
  2. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
    [Google Scholar]
  3. Birtles R. J., Rowbotham T. J., Raoult D., Harrison T. G. 1996; Phylogenetic diversity of intra-amoebal legionellae as revealed by 16S rRNA gene sequence comparison. Microbiology 142:3525–3530 [CrossRef]
    [Google Scholar]
  4. Brenner D. J., Steigerwalt A. G., McDade J. E. 1979; Classification of the Legionnaires' disease bacterium: Legionella pneumophila , genus novum, species nova, of the family Legionellaceae , familia nova. Ann Intern Med 90:656–658 [CrossRef]
    [Google Scholar]
  5. Brenner D. J., Steigerwalt A. G., Epple P., Bibb W. F., McKinney R. M., Starnes R. W., Colville J. M., Selander R. K., Edelstein P. H., Moss C. W. 1988; Legionella pneumophila serogroup Lansing 3 isolated from a patient with fatal pneumonia, and descriptions of L.pneumophila subsp. pneumophila subsp. nov., L. pneumophila subsp. fraseri subsp. nov., and L. pneumophila subsp. pascullei subsp. nov. . J Clin Microbiol 26:1695–1703
    [Google Scholar]
  6. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef]
    [Google Scholar]
  7. Dey R., Bodennec J., Mameri M. O., Pernin P. 2009; Free-living freshwater amoebae differ in their susceptibility to the pathogenic bacterium Legionella pneumophila . FEMS Microbiol Lett 290:10–17 [CrossRef]
    [Google Scholar]
  8. Diederen B. M. 2008; Legionella spp. and Legionnaires' disease. J Infect 56:1–12 [CrossRef]
    [Google Scholar]
  9. Diogo A., Verssimo A., Nobre M. F., Da Costa M. S. 1999; Usefulness of fatty acid composition for differentiation of Legionella species. J Clin Microbiol 37:2248–2254
    [Google Scholar]
  10. Euzéby J. P. 2009 List of Prokaryotic names with Standing in Nomenclature ; http://www.bacterio.cict.fr/l/legionella.html
    [Google Scholar]
  11. Fabbi M., Pastoris M. C., Scanziani E., Magnino S., Di M. L. 1998; Epidemiological and environmental investigations of Legionella pneumophila infection in cattle and case report of fatal pneumonia in a calf. J Clin Microbiol 36:1942–1947
    [Google Scholar]
  12. Feddersen A., Meyer H. G., Matthes P., Bhakdi S., Husmann M. 2000; GyrA sequence-based typing of Legionella . Med Microbiol Immunol (Berl) 189:7–11 [CrossRef]
    [Google Scholar]
  13. Fry N. K., Afshar B., Bellamy W., Underwood A. P., Ratcliff R. M., Harrison T. G. 2007; Identification of Legionella spp. by 19 European reference laboratories: results of the European Working Group for Legionella Infections External Quality Assessment Scheme using DNA sequencing of the macrophage infectivity potentiator gene and dedicated online tools. Clin Microbiol Infect 13:1119–1124 [CrossRef]
    [Google Scholar]
  14. Helbig J. H., Lück P. C., Kunz B., Bubert A. 2006; Evaluation of the Duopath Legionella lateral flow assay for identification of Legionella pneumophila and Legionella species culture isolates. Appl Environ Microbiol 72:4489–4491 [CrossRef]
    [Google Scholar]
  15. International Organization for Standardization 1998; ISO 11731. Water Quality – Detection and enumeration of Legionella . http://www.iso.org/iso/home.html
    [Google Scholar]
  16. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  17. Ko K. S., Lee H. K., Park M. Y., Lee K. H., Yun Y. J., Woo S. Y., Miyamoto H., Kook Y. H. 2002; Application of RNA polymerase beta-subunit gene ( rpoB ) sequences for the molecular differentiation of Legionella species. J Clin Microbiol 40:2653–2658 [CrossRef]
    [Google Scholar]
  18. Kuroki H., Miyamoto H., Fukuda K., Iihara H., Kawamura Y., Ogawa M., Wang Y., Ezaki T., Taniguchi H. 2007; Legionella impletisoli sp. nov. and Legionella yabuuchiae sp. nov., isolated from soils contaminated with industrial wastes in Japan. Syst Appl Microbiol 30:273–279 [CrossRef]
    [Google Scholar]
  19. La Scola B., Birtles R. J., Greub G., Harrison T. J., Ratcliff R. M., Raoult D. 2004; Legionella drancourtii sp. nov., a strictly intracellular amoebal pathogen. Int J Syst Evol Microbiol 54:699–703 [CrossRef]
    [Google Scholar]
  20. Lück P. C., Schmitt J. W., Hengerer A., Helbig J. H. 1998; Subinhibitory concentrations of antimicrobial agents reduce the uptake of Legionella pneumophila into Acanthamoeba castellanii and U937 cells by altering the expression of virulence-associated antigens. Antimicrob Agents Chemother 42:2870–2876
    [Google Scholar]
  21. Lück P. C., Helbig J., von Baum H., Marre R. 2006; Diagnostics and clinical disease treatment: usefulness of microbiological diagnostic methods for detection of Legionella infections. In Legionella: State of the art 30 years after its recognition pp 15–24 Edited by Cianciotto N. P., Abu Kwaik Y., Edelstein P. H., Fields B. S., Geary D. F., Harrison T. G., Joseph C. A., Ratcliff R. M., Stout J. E., Swanson M. S. Washington, DC: ASM Press;
    [Google Scholar]
  22. Miyamoto H., Yamamoto H., Arima K., Fujii J., Maruta K., Izu K., Shiomori T., Yoshida S. 1997; Development of a new seminested PCR method for detection of Legionella species and its application to surveillance of legionellae in hospital cooling tower water. Appl Environ Microbiol 63:2489–2494
    [Google Scholar]
  23. Park M. Y., Ko K. S., Lee H. K., Park M. S., Kook Y. H. 2003; Legionella busanensis sp. nov., isolated from cooling tower water in Korea. Int J Syst Evol Microbiol 53:77–80 [CrossRef]
    [Google Scholar]
  24. Park M., Yun S. T., Kim M. S., Chun J., Ahn T. I. 2004; Phylogenetic characterization of Legionella -like endosymbiotic X-bacteria in Amoeba proteus : a proposal for ‘ Candidatus Legionella jeonii’ sp. nov.. Environ Microbiol 6:1252–1263 [CrossRef]
    [Google Scholar]
  25. Ratcliff R. M., Lanser J. A., Manning P. A., Heuzenroeder M. W. 1998; Sequence-based classification scheme for the genus Legionella targeting the mip gene. J Clin Microbiol 36:1560–1567
    [Google Scholar]
  26. Rubin C. J., Thollesson M., Kirsebom L. A., Herrmann B. 2005; Phylogenetic relationships and species differentiation of 39 Legionella species by sequence determination of the RNase P RNA gene rnpB . Int J Syst Evol Microbiol 55:2039–2049 [CrossRef]
    [Google Scholar]
  27. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  28. Steinert M., Hentschel U., Hacker J. 2002; Legionella pneumophila : an aquatic microbe goes astray. FEMS Microbiol Rev 26:149–162 [CrossRef]
    [Google Scholar]
  29. Steinert M., Leippe M., Roeder T. 2003; Surrogate hosts: protozoa and invertebrates as models for studying pathogen–host interactions. Int J Med Microbiol 293:321–332 [CrossRef]
    [Google Scholar]
  30. von Baum H., Ewig S., Marre R., Suttorp N., Gonschior S., Welte T., Lück C. 2008; Community-acquired Legionella pneumonia: new insights from the German competence network for community acquired pneumonia. Clin Infect Dis 46:1356–1364 [CrossRef]
    [Google Scholar]
  31. Wadowsky R. M., Yee R. B. 1981; Glycine-containing selective medium for isolation of Legionellaceae from environmental specimens. Appl Environ Microbiol 42:768–772
    [Google Scholar]
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