@article{mbs:/content/journal/ijsem/10.1099/ijs.0.018598-0, author = "Cui, Heng-Lin and Li, Xin-Yi and Gao, Xia and Xu, Xue-Wei and Zhou, Yu-Guang and Liu, Hong-Can and Oren, Aharon and Zhou, Pei-Jin", title = "Halopelagius inordinatus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2010", volume = "60", number = "9", pages = "2089-2093", doi = "https://doi.org/10.1099/ijs.0.018598-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.018598-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "PG, phosphatidylglycerol", keywords = "S-DGD-1, sulfated mannosyl glucosyl diether", keywords = "PGP-Me, phosphatidylglycerol phosphate methyl ester", keywords = "DGD-1, mannosyl glucosyl diether", keywords = "PGS, phosphatidylglycerol sulfate", abstract = "Two extremely halophilic archaea, strains RO5-2T and RO5-14, were isolated from Rudong marine solar saltern in Jiangsu, China. Cells of the two strains were pleomorphic, motile and stained Gram-negative. Colonies were red-pigmented. Strains RO5-2T and RO5-14 were able to grow at 20–50 °C (optimum 37 °C), at 2.6–4.8 M NaCl (optimum 3.4–3.9 M NaCl), at 0.03–0.7 M MgCl2 (optimum 0.5 M MgCl2) and at pH 5.5–8.0 (optimum pH 6.5–7.0). Cells lyse in distilled water and the minimal NaCl concentration to prevent cell lysis was 12 % (w/v). The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two major glycolipids chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1) and mannosyl glucosyl diether (DGD-1). The 16S rRNA gene sequences of strains RO5-2T and RO5-14 showed 93.4–93.8 % similarity to the closest cultivated relative, Halosarcina pallida. The DNA G+C content of strains RO5-2T and RO5-14 was 61.0 mol% and 59.9 mol%, respectively. The DNA–DNA relatedness between strains RO5-2T and RO5-14 was 86.0 %. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strains RO5-2T and RO5-14 represent a novel species in a new genus within the family Halobacteriaceae, for which the name Halopelagius inordinatus gen. nov., sp. nov. is proposed. The type strain is RO5-2T (=CGMCC 1.7739T =JCM 15773T).", }