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Volume 60, Issue 10

sp. nov., a halophilic archaeon from a marine solar saltern, and emended description of the genus Free

Abstract

A halophilic archaeon, strain RO1-6, was isolated from a marine solar saltern in eastern China. Cells of strain RO1-6 were pleomorphic and motile and stained Gram-negative. Strain RO1-6 grew well on complex medium and colonies were red-pigmented. It was able to grow at 20–50 °C (optimum 37 °C), in 2.1–5.1 M NaCl (optimum 3.9 M NaCl), in 0.05–0.70 M MgCl (optimum 0.30 M MgCl) and at pH 6.5–8.0 (optimum pH 7.0). Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 12 % (w/v). The major polar lipids of strain RO1-6 were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two glycolipids that were chromatographically identical to S-DGD-1 and S-DGD. The 16S rRNA gene sequence of strain RO1-6 showed similarities of 96.9 and 96.4 % to those of the type strains of and , respectively, members of the most closely related recognized genera within the family . The DNA G+C content of strain RO1-6 was 61.2 mol%. Phenotypic characterization and phylogenetic analysis revealed that strain RO1-6 is related to and represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is RO1-6 (=CGMCC 1.8711 =JCM 16054).

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Keyword(s): PG, phosphatidylglycerol and PGP-Me, phosphatidylglycerol phosphate methyl ester
SGM
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2010-10-01
2024-04-19
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Halosarcina limi sp. nov., a halophilic archaeon from a marine solar saltern, and emended description of the genus Halosarcina
Int J Syst Evol Microbiol 60, 2462 (2010); https://doi.org/10.1099/ijs.0.018697-0
/content/journal/ijsem/10.1099/ijs.0.018697-0
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