1887

Abstract

A Gram-positive, non-motile, coccoid- to rod-shaped, non-spore-forming bacterium, designated strain YM18-15, was isolated from sea sand and studied using a polyphasic taxonomic approach. Strain YM18-15 grew under both aerobic and anaerobic conditions. The cell-wall peptidoglycan type was A4 and ornithine was the diagnostic diamino acid. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and an unknown phospholipid, MK-8(H) was the major menaquinone and the predominant fatty acids were anteiso-C and C. The DNA G+C content was 74.2 mol%. High 16S rRNA gene sequence similarities (96.3–97.3 %) were found with the sequences of the type strains of the three genera of the family . Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YM18-15 formed a clade with , and . Strain YM18-15 differed from these three type strains in chemotaxonomic characteristics and in 16S rRNA gene signature nucleotides. Based on genetic and chemotaxonomic evidence, it is suggested that strain YM18-15 represents a novel species of a new genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is YM18-15 (=NBRC 106267=KCTC 19750=MBIC 08348).

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2011-01-01
2024-03-29
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References

  1. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distributions of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
    [Google Scholar]
  2. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  3. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  4. Groth I., Schumann P., Schuetze B., Augsten K., Kramer I., Stackebrandt E. 1999; Beutenbergia cavernae gen. nov., sp. nov. an l-lysine-containing actinomycete isolated from a cave. Int J Syst Bacteriol 49:1733–1740 [CrossRef]
    [Google Scholar]
  5. Hamada M., Iino T., Tamura T., Iwami T., Harayama S., Suzuki K. 2009; Serinibacter salmoneus gen. nov., sp. nov. an actinobacterium isolated from the intestinal tract of a fish, and emended descriptions of the families Beutenbergiaceae and Bogoriellaceae . Int J Syst Evol Microbiol 592809–2814 [CrossRef]
    [Google Scholar]
  6. Harper J. J., Davis G. H. G. 1979; Two-dimensional thin-layer chromatography for amino acid analysis of bacterial cell walls. Int J Syst Bacteriol 29:56–58 [CrossRef]
    [Google Scholar]
  7. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press;
    [Google Scholar]
  8. Kumagai M., Fujimoto M., Kuninaka A. 1988; Determination of base composition of DNA by high performance liquid chromatography of its nuclease P1 hydrolysate. Nucleic Acids Symp Ser 19:65–68
    [Google Scholar]
  9. Minnikin D. E., Alshamaony L., Goodfellow M. 1975; Differentiation of Mycobacterium , Nocardia , and related taxa by thin layer chromatographic analysis of whole-cell methanolysates. J Gen Microbiol 88:200–204 [CrossRef]
    [Google Scholar]
  10. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  11. Ogiso H., Suzuki T., Taguchi R. 2008; Development of a reverse-phase liquid chromatography electrospray ionization mass spectrometry method for lipidomics, improving detection of phosphatidic acid and phosphatidylserine. Anal Biochem 375:124–131 [CrossRef]
    [Google Scholar]
  12. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  13. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  14. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  15. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. 1999; Phylogenetic analysis of genus Marinilabilia and related bacteria based on the amino acid sequences of GyrB and emended description of Marinilabilia salmonicolor with Marinilabilia agarovorans as its subjective synonym. Int J Syst Bacteriol 49:1551–1557 [CrossRef]
    [Google Scholar]
  16. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  17. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  18. Uchida K., Kudo T., Suzuki K., Nakase T. 1999; A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45:49–56 [CrossRef]
    [Google Scholar]
  19. von Wintzingerode F, Göbel U. B, Siddiqui R. A., Rösick U, Schumann P., Frühling A, Rohde M., Pukall R., Stackebrandt E. 2001; Salana multivorans gen. nov., sp. nov., a novel actinobacterium isolated from an anaerobic bioreactor and capable of selenate reduction. Int J Syst Evol Microbiol 51:1653–1661 [CrossRef]
    [Google Scholar]
  20. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
    [Google Scholar]
  21. Yokota A., Tamura T., Nishii T., Hasegawa T. 1993; Kineococcus aurantiacus gen. nov., sp. nov. a new aerobic, gram-positive, motile coccus with meso -diaminopimelic acid and arabinogalactan in the cell wall. Int J Syst Bacteriol 4352–57 [CrossRef]
    [Google Scholar]
  22. Zhi X.-Y., Li W.-J., Stackebrandt E. 2009; An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria , with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol 59:589–608 [CrossRef]
    [Google Scholar]
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