@article{mbs:/content/journal/ijsem/10.1099/ijs.0.020131-0, author = "Iino, Takao and Mori, Koji and Suzuki, Ken-ichiro", title = "Methanospirillum lacunae sp. nov., a methane-producing archaeon isolated from a puddly soil, and emended descriptions of the genus Methanospirillum and Methanospirillum hungatei", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2010", volume = "60", number = "11", pages = "2563-2566", doi = "https://doi.org/10.1099/ijs.0.020131-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.020131-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A mesophilic, hydrogenotrophic methanogen, designated strain Ki8-1T, was isolated from soil. Cells were strictly anaerobic, Gram-stain-negative, non-sporulating, motile by means of a single flagellum or tufted flagella, and curved or wavy rod-shaped (11–25 μm long). The temperature and pH for optimum growth were 30 °C and 7.5. The strain grew best in basal medium without the addition of NaCl. Methane was produced from H2 and formate. Acetate or yeast extract was required for growth. The G+C content of the genomic DNA of strain Ki8-1T was 45.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Ki8-1T was a member of the genus Methanospirillum and showed 95.1 % sequence similarity to Methanospirillum hungatei NBRC 100397T. On the basis of its phenotypic characteristics and phylogenetic position, strain Ki8-1T is considered to represent a novel species of the genus Methanospirillum, for which the name Methanospirillum lacunae sp. nov. is proposed. The type strain is Ki8-1T (NBRC 104920T =JCM 16384T =DSM 22751T). Emended descriptions of the genus Methanospirillum and of Methanospirillum hungatei are also provided.", }