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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 60, Issue 12

Differentiation of species using partial RNA polymerase -subunit () gene sequences Free

Abstract

Partial RNA polymerase -subunit gene () sequences (315 bp) were determined and used to differentiate the type strains of 23 species of the genus . The sequences were compared with those of the partial (604 bp) and 16S rRNA genes (1475 or 1495 bp). The gene sequences showed nucleotide sequence similarities ranging from 84.1 % to 99.0 %, while the similarities of the sequences ranged from 78.5 % to 99.7 % and the 16S rRNA gene sequence similarities ranged from 89.4 % to 99.2 %. The phylogenetic trees constructed from the sequences of these three genes showed similar clustering patterns, with the exception of several species. The , subsp. subsp. and groups were more clearly differentiated in the partial and gene sequence trees than they were in the 16S rRNA gene tree. Based on sequence similarities and tree topologies, the newly determined gene sequences are suitable molecular markers for the differentiation of species of the genus and support various other molecular tools used to determine the relationships among species of this genus.

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Keyword(s): HGT, horizontal gene transfer , ILD, incongruence length difference , ML, maximum-likelihood , NJ, neighbour-joining and SH, Shimodaira-Hasegawa
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2010-12-01
2024-04-20
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Differentiation of Bifidobacterium species using partial RNA polymerase β-subunit (rpoB) gene sequences
Int J Syst Evol Microbiol 60, 2697 (2010); https://doi.org/10.1099/ijs.0.020339-0
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