RT Journal Article SR Electronic(1) A1 Lucena, Teresa A1 Pascual, Javier A1 Giordano, Assunta A1 Gambacorta, Agata A1 Garay, Esperanza A1 Arahal, David R. A1 Macián, M. Carmen A1 Pujalte, María J.YR 2010 T1 Euzebyella saccharophila gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae JF International Journal of Systematic and Evolutionary Microbiology, VO 60 IS 12 SP 2871 OP 2876 DO https://doi.org/10.1099/ijs.0.020875-0 PB Microbiology Society, SN 1466-5034, AB Strain 7SM30T , an aerobic marine, Gram-negative, heterotrophic and yellow- to orange-pigmented bacterium isolated from seawater from Castellón, Spain, was characterized using a polyphasic approach. Analysis of the 16S rRNA gene sequence showed that the isolate represented a novel lineage within the family Flavobacteriaceae. The most closely related genera were Pseudozobellia, Zobellia and Kriegella. Cells of strain 7SM30T were non-motile rods that required sea salts for growth, used a wide variety of carbohydrates as sole carbon and energy sources and, unlike species of the genera Pseudozobellia and Zobellia, did not possess flexirubin-type pigment or hydrolyse agar. Strain 7SM30T contained MK6 as the sole respiratory quinone. Phosphatidylethanolamine (PE) was the only identifiable polar lipid, although other lipids were also detected. The predominant cellular fatty acids were saturated C15 and monounsaturated C15. The DNA G+C content was around 40 mol%. On the basis of extensive phenotypic and phylogenetic comparative analysis, it is concluded that the new strain represents a novel genus and species, for which the name Euzebyella saccharophila gen. nov., sp. nov., is proposed. The type strain of the type species is 7SM30T (=CECT 7477T=KCTC 22655T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.020875-0