RT Journal Article SR Electronic(1) A1 Huo, Ying-Yi A1 Xu, Xue-Wei A1 Liu, Sheng-Pan A1 Cui, Heng-Lin A1 Li, Xue A1 Wu, MinYR 2011 T1 Sphingomonas rubra sp. nov., isolated from bioreactor wastewater JF International Journal of Systematic and Evolutionary Microbiology, VO 61 IS 5 SP 1028 OP 1032 DO https://doi.org/10.1099/ijs.0.020958-0 PB Microbiology Society, SN 1466-5034, AB A Gram-reaction-negative, rod-shaped, motile, neutrophilic bacterium, designated strain BH3T, was isolated from wastewater of a sequential batch reactor treating wastewater taken from a leather plant. The isolate grew in 0–8 % (w/v) NaCl, at pH 6–9 and at 4–45 °C. Chemotaxonomic analysis showed that strain BH3T had characteristics typical of members of the genus Sphingomonas, such as the presence of sphingolipids, Q-10 and 2-hydroxymyristic acid and the absence of 3-hydroxy fatty acids. The presence of C18 : 1ω7c (39.2 %) and C16 : 0 (11.2 %) as major fatty acids, C14 : 0 2-OH (20.6 %) as the major 2-hydroxy fatty acid and homospermidine as the major polyamine indicated that strain BH3T belonged to the genus Sphingomonas sensu stricto. The genomic DNA G+C content of strain BH3T was 65.6 mol%. 16S rRNA gene sequence similarities between the isolate and closely related members of the genus Sphingomonas sensu stricto ranged from 92.6 to 97.3 %, the highest sequence similarities being to Sphingomonas melonis DSM 14444T (97.3 %) and Sphingomonas aquatilis DSM 15581T (97.3 %). Based on its phenotypic characteristics and the results of DNA–DNA hybridization studies and 16S rRNA gene sequence comparisons, strain BH3T represents a novel species of the genus Sphingomonas sensu stricto, for which the name Sphingomonas rubra sp. nov. is proposed. The type strain is BH3T ( = CGMCC 1.9113T  = JCM 16230T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.020958-0