1887

Abstract

A Gram-negative, rod-shaped, strictly aerobic bacterium, strain 2622, was isolated from gamma-irradiated soil sampled from the Taklimakan desert in Xinjiang, China. Phylogenetic analyses showed that strain 2622 formed a distinct lineage in the family and shared 91.7 and 90.1 % 16S rRNA gene sequence similarity with its closest relatives, the type strains of and , respectively. The DNA G+C content of strain 2622 was 71.4 mol% and the isoprenoid quinone was ubiquinone Q-10. Based on phenotypic and chemotaxonomic data and phylogenetic analysis, strain 2622 is considered to represent a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is strain 2622 ( = CCTCC AB 208152  = KCTC 22436).

Funding
This study was supported by the:
  • Ministry of Science and Technology of the People’s Republic of China (Award 2005DKA21208)
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2011-05-01
2024-04-16
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References

  1. An H., Zhang L., Tang Y., Luo X., Sun T., Li Y., Wang Y., Dai J., Fang C. 2009; Skermanella xinjiangensis sp. nov., isolated from the desert of Xinjiang, China. Int J Syst Evol Microbiol 59:1531–1534 [View Article][PubMed]
    [Google Scholar]
  2. Biebl H., Allgaier M., Tindall B. J., Koblizek M., Lünsdorf H., Pukall R., Wagner-Döbler I. 2005; Dinoroseobacter shibae gen. nov., sp. nov., a new aerobic phototrophic bacterium isolated from dinoflagellates. Int J Syst Evol Microbiol 55:1089–1096 [View Article][PubMed]
    [Google Scholar]
  3. Buczolits S., Denner E. B. M., Vybiral D., Wieser M., Kämpfer P., Busse H. J. 2002; Classification of three airborne bacteria and proposal of Hymenobacter aerophilus sp. nov.. Int J Syst Evol Microbiol 52:445–456[PubMed]
    [Google Scholar]
  4. Choi D. H., Hwang C. Y., Cho B. C. 2009; Pelagibius litoralis gen. nov., sp. nov., a marine bacterium in the family Rhodospirillaceae isolated from coastal seawater. Int J Syst Evol Microbiol 59:818–823 [View Article][PubMed]
    [Google Scholar]
  5. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  6. Cox M. M., Battista J. R. 2005; Deinococcus radiodurans – the consummate survivor. Nat Rev Microbiol 3:882–892 [View Article][PubMed]
    [Google Scholar]
  7. Díaz-Cárdenas C., Patel B. K., Baena S. 2010; Tistlia consotensis gen. nov., sp. nov., an aerobic, chemoheterotrophic, free-living, nitrogen-fixing alphaproteobacterium, isolated from a Colombian saline spring. Int J Syst Evol Microbiol 60:1437–1443 [View Article][PubMed]
    [Google Scholar]
  8. Eckert B., Weber O. B., Kirchhof G., Halbritter A., Stoffels M., Hartmann A. 2001; Azospirillum doebereinerae sp. nov., a nitrogen-fixing bacterium associated with the C4-grass Miscanthus . Int J Syst Evol Microbiol 51:17–26
    [Google Scholar]
  9. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  10. Fredrickson J. K., Li S. M., Gaidamakova E. K., Matrosova V. Y., Zhai M., Sulloway H. M., Scholten J. C., Brown M. G., Balkwill D. L., Daly M. J. 2008; Protein oxidation: key to bacterial desiccation resistance?. ISME J 2:393–403 [View Article][PubMed]
    [Google Scholar]
  11. Garrity G. M., Bell J. A., Lilburn T. 2005; Family I. Rhodospirillaceae Pfennig and Trüper 1971, 17AL . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 2C pp. 1–40 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  12. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  13. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  14. Kodama Y., Stiknowati L. I., Ueki A., Ueki K., Watanabe K. 2008; Thalassospira tepidiphila sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from seawater. Int J Syst Evol Microbiol 58:711–715 [View Article][PubMed]
    [Google Scholar]
  15. Lai Q., Yuan J., Gu L., Shao Z. 2009a; Marispirillum indicum gen. nov., sp. nov., isolated from a deep-sea environment. Int J Syst Evol Microbiol 59:1278–1281 [View Article][PubMed]
    [Google Scholar]
  16. Lai Q., Yuan J., Wu C., Shao Z. 2009b; Oceanibaculum indicum gen. nov., sp. nov., isolated from deep seawater of the Indian Ocean. Int J Syst Evol Microbiol 59:1733–1737 [View Article][PubMed]
    [Google Scholar]
  17. Lakshmi K. V. N. S., Sasikala Ch., Ashok G. V., Chandrasekaran R., Ramana Ch. V. 2011; Phaeovibrio sulfidiphilus gen. nov., sp. nov., phototrophic alphaproteobacteria isolated from brackish water. Int J Syst Evol Microbiol 61:828–833 [View Article][PubMed]
    [Google Scholar]
  18. Liu C., Wu Y., Li L., Ma Y., Shao Z. 2007; Thalassospira xiamenensis sp. nov. and Thalassospira profundimaris sp. nov.. Int J Syst Evol Microbiol 57:316–320 [View Article][PubMed]
    [Google Scholar]
  19. Liu Y., Jin J. H., Liu Y. H., Zhou Y. G., Liu Z. P. 2010; Dongia mobilis gen. nov., sp. nov., a new member of the family Rhodospirillaceae isolated from a sequencing batch reactor for treatment of malachite green effluent. Int J Syst Evol Microbiol 60:2780–2785 [View Article][PubMed]
    [Google Scholar]
  20. López-López A., Pujalte M. J., Benlloch S., Mata-Roig M., Rosselló-Mora R., Garay E., Rodríguez-Valera F. 2002; Thalassospira lucentensis gen. nov., sp. nov., a new marine member of the α-Proteobacteria . Int J Syst Evol Microbiol 52:1277–1283 [View Article][PubMed]
    [Google Scholar]
  21. Mack E. E., Mandelco L., Woese C. R., Madigan M. T. 1993; Rhodospirillum sodomense, sp. nov., a Dead Sea Rhodospirillum species. Arch Microbiol 160:363–371 [View Article]
    [Google Scholar]
  22. Mattimore V., Battista J. R. 1996; Radioresistance of Deinococcus radiodurans: functions necessary to survive ionizing radiation are also necessary to survive prolonged desiccation. J Bacteriol 178:633–637[PubMed]
    [Google Scholar]
  23. Mehnaz S., Lazarovits G. 2006; Inoculation effects of Pseudomonas putida, Gluconacetobacter azotocaptans, and Azospirillum lipoferum on corn plant growth under greenhouse conditions. Microb Ecol 51:326–335 [View Article][PubMed]
    [Google Scholar]
  24. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  25. Pfennig N., Lünsdorf H., Süling J., Imhoff J. F. 1997; Rhodospira trueperi gen. nov., spec. nov., a new phototrophic proteobacterium of the alpha group. Arch Microbiol 168:39–45 [View Article][PubMed]
    [Google Scholar]
  26. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov.. Int J Syst Bacteriol 46:1088–1092 [View Article][PubMed]
    [Google Scholar]
  27. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  28. Sghaier H., Ghedira K., Benkahla A., Barkallah I. 2008; Basal DNA repair machinery is subject to positive selection in ionizing-radiation-resistant bacteria. BMC Genomics 9:297 [View Article][PubMed]
    [Google Scholar]
  29. Sly L. I., Stackebrandt E. 1999; Description of Skermanella parooensis gen. nov., sp. nov. to accommodate Conglomeromonas largomobilis subsp. parooensis following the transfer of Conglomeromonas largomobilis subsp. largomobilis to the genus Azospirillum . Int J Syst Bacteriol 49:541–544 [View Article]
    [Google Scholar]
  30. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 611–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  31. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  32. Tarrand J. J., Krieg N. R., Döbereiner J. 1978; A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov.. Can J Microbiol 24:967–980 [View Article][PubMed]
    [Google Scholar]
  33. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  34. Urios L., Michotey V., Intertaglia L., Lesongeur F., Lebaron P. 2008; Nisaea denitrificans gen. nov., sp. nov. and Nisaea nitritireducens sp. nov., two novel members of the class Alphaproteobacteria from the Mediterranean Sea. Int J Syst Evol Microbiol 58:2336–2341 [View Article][PubMed]
    [Google Scholar]
  35. Wang Y. X., Liu J. H., Zhang X. X., Chen Y. G., Wang Z. G., Chen Y., Li Q. Y., Peng Q., Cui X. L. 2009; Fodinicurvata sediminis gen. nov., sp. nov. and Fodinicurvata fenggangensis sp. nov., poly-β-hydroxybutyrate-producing bacteria in the family Rhodospirillaceae . Int J Syst Evol Microbiol 59:2575–2581 [View Article][PubMed]
    [Google Scholar]
  36. Weon H. Y., Kim B. Y., Hong S. B., Joa J. H., Nam S. S., Lee K. H., Kwon S. W. 2007; Skermanella aerolata sp. nov., isolated from air, and emended description of the genus Skermanella . Int J Syst Evol Microbiol 57:1539–1542 [CrossRef]
    [Google Scholar]
  37. Wilson K. 1987; Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology pp. 2.4.1–2.4.2 Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. New York: Wiley;
    [Google Scholar]
  38. Xie C. H., Yokota A. 2003; Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49:345–349 [View Article][PubMed]
    [Google Scholar]
  39. Xie C. H., Yokota A. 2005; Azospirillum oryzae sp. nov., a nitrogen-fixing bacterium isolated from the roots of the rice plant Oryza sativa . Int J Syst Evol Microbiol 55:1435–1438 [CrossRef]
    [Google Scholar]
  40. Yoon J. H., Kang S.-J., Park S., Oh T.-K. 2007; Caenispirillum bisanense gen. nov., sp. nov., isolated from sludge of a dye works. Int J Syst Evol Microbiol 57:1217–1221 [View Article][PubMed]
    [Google Scholar]
  41. Zhang L., Yang Q., Luo X., Fang C., Zhang Q., Tang Y. 2007; Knockout of crtB or crtI gene blocks the carotenoid biosynthetic pathway in Deinococcus radiodurans R1 and influences its resistance to oxidative DNA-damaging agents due to change of free radicals scavenging ability. Arch Microbiol 188:411–419 [View Article][PubMed]
    [Google Scholar]
  42. Zhang G. I., Hwang C. Y., Cho B. C. 2008; Thalassobaculum litoreum gen. nov., sp. nov., a member of the family Rhodospirillaceae isolated from coastal seawater. Int J Syst Evol Microbiol 58:479–485 [View Article][PubMed]
    [Google Scholar]
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