1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 61, Issue 9

sp. nov., isolated from a traditional Korean food Free

Abstract

An actinobacterial strain, designated 72, was isolated from a traditional salt-fermented seafood in Korea. Colonies were coral red and cells were Gram-reaction-positive, non-motile rods. Strain 72 grew with 0–10 % (w/v) NaCl, at pH 7–10 and at 15–37 °C. Optimum growth conditions were 2 % NaCl, pH 7.0 and 30 °C. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain 72 belonged to the genus . The major cellular fatty acids (>5 %) were C, summed feature 3 (comprising Cω6 and/or Cω7), 10-methyl C, C, C and Cω9. 16S rRNA gene sequence analysis and DNA–DNA hybridization, coupled with physiological and biochemical tests, revealed genotypic and phenotypic differences between strain 72 and other members of the genus . Based on these data, strain 72 represents a novel species, for which the name sp. nov. is proposed. The type strain is 72 ( = JCM 16360  = KACC 21126).

  • Published Online:
Funding
This study was supported by the:
  • TDPAF (Technology Development Program for Agriculture and Forestry
© 2011 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.021501-0
2011-09-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/9/2254.html?itemId=/content/journal/ijsem/10.1099/ijs.0.021501-0&mimeType=html&fmt=ahah

References

  1. Bae J. W., Rhee S. K., Park J. R., Chung W. H., Nam Y. D., Lee I., Kim H., Park Y. H. 2005; Development and evaluation of genome-probing microarrays for monitoring lactic acid bacteria. Appl Environ Microbiol 71:8825–8835 [View Article][PubMed]
     [Google Scholar]
  2. Baker G. C., Smith J. J., Cowan D. A. 2003; Review and re-analysis of domain-specific 16S primers. J Microbiol Methods 55:541–555 [View Article][PubMed]
     [Google Scholar]
  3. Bousfield I. J., Keddie R. M., Dando T. R., Shaw S. 1985; Simple rapid methods of cell wall analysis as an aid in the identification of aerobic coryneform bacteria. In Chemical Methods in Bacterial Systematics pp. 221–236 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
     [Google Scholar]
  4. Buck J. D. 1982; Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993[PubMed]
     [Google Scholar]
  5. Colquhoun J. A., Mexson J., Goodfellow M., Ward A. C., Horikoshi K., Bull A. T. 1998; Novel rhodococci and other mycolate actinomycetes from the deep sea. Antonie van Leeuwenhoek 74:27–40 [View Article][PubMed]
     [Google Scholar]
  6. Duckworth A. W., Grant S., Grant W. D., Jones B. E., Meijer D. 1998; Dietzia natronolimnaios sp. nov., a new member of the genus Dietzia isolated from an east African soda lake. Extremophiles 2:359–366 [View Article][PubMed]
     [Google Scholar]
  7. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
     [Google Scholar]
  8. Gonzalez J. M., Saiz-Jimenez C. 2002; A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4:770–773 [View Article][PubMed]
     [Google Scholar]
  9. Goodfellow M. 1986; Genus Rhodococcus Zopf 1891, 28AL . In Bergey’s Manual of Systematic Bacteriology vol. 2 pp. 1472–1481 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
     [Google Scholar]
  10. Jones A. L., Koerner R. J., Natarajan S., Perry J. D., Goodfellow M. 2008; Dietzia papillomatosis sp. nov., a novel actinomycete isolated from the skin of an immunocompetent patient with confluent and reticulated papillomatosis. Int J Syst Evol Microbiol 58:68–72 [View Article][PubMed]
     [Google Scholar]
  11. Kämpfer P., Langer S., Martin E., Jäckel U., Busse H. J. 2010; Dietzia aerolata sp. nov., isolated from the air of a duck barn, and emended description of the genus Dietzia Rainey et al. 1995. Int J Syst Evol Microbiol 60:393–396 [View Article][PubMed]
     [Google Scholar]
  12. Kluge A. G., Farris F. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [View Article]
     [Google Scholar]
  13. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
     [Google Scholar]
  14. Lechevalier M. P. 1968; Identification of aerobic actinomycetes of clinical importance. J Lab Clin Med 71:934–944[PubMed]
     [Google Scholar]
  15. Li J., Zhao G.-Z., Zhang Y.-Q., Klenk H.-P., Pukall R., Qin S., Xu L.-H., Li W.-J. 2008; Dietzia schimae sp. nov. and Dietzia cercidiphylli sp. nov., from surface-sterilized plant tissues. Int J Syst Evol Microbiol 58:2549–2554 [View Article][PubMed]
     [Google Scholar]
  16. Mayilraj S., Suresh K., Kroppenstedt R. M., Saini H. S. 2006; Dietzia kunjamensis sp. nov., isolated from the Indian Himalayas. Int J Syst Evol Microbiol 56:1667–1671 [View Article][PubMed]
     [Google Scholar]
  17. Minnikin D. E., Alshamaony L., Goodfellow M. 1975; Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88:200–204[PubMed] [CrossRef]
     [Google Scholar]
  18. Nesterenko O. A., Nogina T. M., Kasumova S. A., Kvasnikov E. I., Batrakov S. G. 1982; Rhodococcus luteus nom. nov. and Rhodococcus maris nom. nov.. Int J Syst Bacteriol 32:1–14 [View Article]
     [Google Scholar]
  19. Rainey F. A., Klatte S., Kroppenstedt R. M., Stackebrandt E. 1995; Dietzia, a new genus including Dietzia maris comb. nov., formerly Rhodococcus maris . Int J Syst Bacteriol 45:32–36 [View Article][PubMed]
     [Google Scholar]
  20. Roh S. W., Sung Y., Nam Y. D., Chang H. W., Kim K. H., Yoon J. H., Jeon C. O., Oh H. M., Bae J. W. 2008; Arthrobacter soli sp. nov., a novel bacterium isolated from wastewater reservoir sediment. J Microbiol 46:40–44 [View Article][PubMed]
     [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  22. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  23. Suh H. K., Yoon S. S. 1987; A study on the regional characteristics of Korean chotkal. Korean J Dietary Cult 2:45–54
     [Google Scholar]
  24. Takami H., Inoue A., Fuji F., Horikoshi K. 1997; Microbial flora in the deepest sea mud of the Mariana Trench. FEMS Microbiol Lett 152:279–285 [View Article][PubMed]
     [Google Scholar]
  25. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
     [Google Scholar]
  26. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  27. Tindall B. J. 1990; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [View Article]
     [Google Scholar]
  28. Tittsler R. P., Sandholzer L. A. 1936; The use of semi-solid agar for the detection of bacterial motility. J Bacteriol 31:575–580[PubMed]
     [Google Scholar]
  29. Xin H., Itoh T., Zhou P., Suzuki K., Kamekura M., Nakase T. 2000; Natrinema versiforme sp. nov., an extremely halophilic archaeon from Aibi salt lake, Xinjiang, China. Int J Syst Evol Microbiol 50:1297–1303 [View Article][PubMed]
     [Google Scholar]
  30. Yassin A. F., Hupfer H., Schaal K. P. 2006; Dietzia cinnamea sp. nov., a novel species isolated from a perianal swab of a patient with a bone marrow transplant. Int J Syst Evol Microbiol 56:641–645 [View Article][PubMed]
     [Google Scholar]
  31. Yumoto I., Nakamura A., Iwata H., Kojima K., Kusumoto K., Nodasaka Y., Matsuyama H. 2002; Dietzia psychralcaliphila sp. nov., a novel, facultatively psychrophilic alkaliphile that grows on hydrocarbons. Int J Syst Evol Microbiol 52:85–90[PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.021501-0
Loading
Dietzia alimentaria sp. nov., isolated from a traditional Korean food
Int J Syst Evol Microbiol 61, 2254 (2011); https://doi.org/10.1099/ijs.0.021501-0
/content/journal/ijsem/10.1099/ijs.0.021501-0
/content/journal/ijsem/10.1099/ijs.0.021501-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error