@article{mbs:/content/journal/ijsem/10.1099/ijs.0.022228-0, author = "Zhang, Guo Xia and Ren, Sui Zhou and Xu, Mei Ying and Zeng, Guo Qu and Luo, Hui Dong and Chen, Jin Lin and Tan, Zhi Yuan and Sun, Guo Ping", title = "Rhizobium borbori sp. nov., aniline-degrading bacteria isolated from activated sludge", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2011", volume = "61", number = "4", pages = "816-822", doi = "https://doi.org/10.1099/ijs.0.022228-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.022228-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Three aniline-degrading bacteria, strains DN316T, DN316-1 and DN365, were isolated from activated sludge. According to 16S rRNA gene sequence-based phylogenetic analysis, the isolates belonged to the genus Rhizobium, with Rhizobium ( = Agrobacterium) radiobacter LMG 140T as the closest relative, with 96.5 % sequence similarity. Phylogenetic analysis of the representative strain DN316T using sequences of the glnA, thrC and recA genes and the 16S–23S intergenic spacer region confirmed the phylogenetic arrangement obtained from analysis of the 16S rRNA gene. DNA–DNA relatedness between DN316T and R. radiobacter LMG 140T was 43.7 %, clearly indicating that the representative strain DN316T represents a novel species. Phenotypic and biochemical characterization of the isolates and insertion sequence-PCR fingerprinting patterns showed several distinctive features that differentiated them from closely related species. The major components of the cellular fatty acids were C18 : 1ω7c (57.10 %), C16 : 0 (11.31 %) and C19 : 0 cyclo ω8c (10.13 %). Based on our taxonomic analysis, the three isolates from activated sludge represent a novel species of the genus Rhizobium, for which the name Rhizobium borbori sp. nov. is proposed. The type strain is DN316T ( = CICC 10378T  = LMG 23925T).", }