@article{mbs:/content/journal/ijsem/10.1099/ijs.0.02276-0, author = "Mechichi, Tahar and Stackebrandt, Erko and Fuchs, Georg", title = "Alicycliphilus denitrificans gen. nov., sp. nov., a cyclohexanol-degrading, nitrate-reducing β-proteobacterium", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2003", volume = "53", number = "1", pages = "147-152", doi = "https://doi.org/10.1099/ijs.0.02276-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.02276-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A facultatively denitrifying bacterium, strain K601T, was isolated at 30 °C from a municipal sewage plant on cyclohexanol as sole carbon source and nitrate as electron acceptor. Under aerobic conditions this strain used acetate, fumarate, lactate, pyruvate, crotonate, indole, glucose, vanillate, 4-hydroxybenzoate, m-cresol, o-cresol and p-cresol. Under denitrifying conditions the strain used cyclohexanol, cyclohexanone, 1,3-cyclohexanedione, 2-cyclohexenone, 1,3-cyclohexanediol (cis and trans), monocarboxylic acids (C2–C7), adipate, pimelate, 5-oxocaproate, citrate, 2-oxoglutarate, succinate, malate, crotonate, lactate, pyruvate and fumarate. Cells were short rods, 0·6 μm wide and 1–2 μm long, motile, non-spore-forming, Gram-negative, and catalase- and oxidase-positive. Strain K601T used nitrate, nitrite and oxygen as electron acceptors, but not sulfate, sulfite or fumarate. The DNA G+C content of strain K601T was 66 mol%. Phylogenetic analysis, based on 16S rDNA sequencing, showed that strain K601T represents a separate lineage of the family Comamonadaceae in the β-subclass of Proteobacteria. Based on the high 16S rDNA sequence divergence and phenotypic characteristics, the name Alicycliphilus denitrificans gen. nov., sp. nov. is proposed for this strain. The type strain is K601T (=DSM 14773T =CIP 107495T).", }