1887

Abstract

The microflora isolated from food-packaging board is dominated by paenibacilli; a number of these micro-organisms have been characterized using a polyphasic approach. The highest 16S rRNA gene similarity was found between these isolates and ATCC 35681 (97·7 %). The main fatty acid of the paperboard isolates was C (34–45 %); straight-chain fatty acids made up 41–60 % of the total cellular fatty acids, thus distinguishing these strains from other species. The paperboard isolates produced cyclodextrins from starch. The spore surface had a characteristic ribbed ornamentation. Spores and vegetative cells frequently had pilus-like appendages. Based on phylogenetic data and phenotypic and chemotaxonomic characteristics, it is proposed that the isolates represent a novel species, sp. nov., with IS 1 (=DSM 14472=CCUG 45566) as the type strain.

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2003-09-01
2024-03-28
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References

  1. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef]
    [Google Scholar]
  2. Clesceri L. S., Greenberg A. E., Eaton A. D. 1998; Microbiological examination – heterotrophic plate count. In Standard Methods for the Examination of Water and Wastewater , 20th edn. pp. 9-34–9-36 Washington, DC: American Public Health Association;
    [Google Scholar]
  3. Elo S., Suominen I., Kämpfer P., Juhanoja J., Salkinoja-Salonen M., Haahtela K. 2001; Paenibacillus borealis sp. nov., a nitrogen-fixing species isolated from spruce forest humus in Finland. Int J Syst Evol Microbiol 51:535–545
    [Google Scholar]
  4. Felsenstein J. 1993 phylip (phylogenetic inference package) version 3.5.1 Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  5. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol 3 pp 21–132Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  6. Kozuka S., Tochikubo K. 1985; Properties and origin of filamentous appendages on spores of Bacillus cereus . Microbiol Immunol 29:21–37 [CrossRef]
    [Google Scholar]
  7. Maidak B. L., Cole J. R., Parker C. T. Jr11 other authors 1999; A new version of the RDP (Ribosomal Database Project. Nucleic Acids Res 27:171–173 [CrossRef]
    [Google Scholar]
  8. Mizuki E., Ohba M., Ichimatsu T., Hwang S.-H., Higuchi K., Saitoh H., Akao T. 1998; Unique appendages associated with spores of Bacillus cereus isolates. J Basic Microbiol 38:33–39 [CrossRef]
    [Google Scholar]
  9. Murphy J. A., Campbell L. L. 1969; Surface features of Bacillus polymyxa spores as revealed by scanning electron microscopy. J Bacteriol 98:737–743
    [Google Scholar]
  10. Pirttijärvi T. S. M., Graeffe T. H., Salkinoja-Salonen M. S. 1996; Bacterial contaminants in liquid packaging boards: assessment of potential for food spoilage. J Appl Bacteriol 81:445–458
    [Google Scholar]
  11. Pirttijärvi T. S. M., Wahlström G., Rainey F. A., Saris P. E. J., Salkinoja-Salonen M. S. 2001; Inhibition of bacilli in industrial starches by nisin. J Ind Microbiol Biotechnol 26:107–114 [CrossRef]
    [Google Scholar]
  12. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [CrossRef]
    [Google Scholar]
  13. Seldin L., Van Elsas J. D., Penido E. G. C. 1984; Bacillus azotofixans sp. nov., a nitrogen-fixing species from Brazilian soils and grass roots. Int J Syst Bacteriol 34:451–456 [CrossRef]
    [Google Scholar]
  14. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp 607–654Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  15. Suominen I., Suihko M.-L., Salkinoja-Salonen M. 1997; Microscopic study of migration of microbes in food-packaging paper and board. J Ind Microbiol Biotechnol 19:104–113 [CrossRef]
    [Google Scholar]
  16. TAPPI 1990 Bacteriological examination of paper and board TAPPI standard T449 om-90 Atlanta: Technical Association of Pulp and Paper;
    [Google Scholar]
  17. van der Maarel M. J. E. C., Veen A., Wijbenga D. J. 2000; Paenibacillus granivorans sp. nov., a new Paenibacillus species which degrades native potato starch granules. Syst Appl Microbiol 23:344–348 [CrossRef]
    [Google Scholar]
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