1887

Abstract

Gregarines are thought to be deep-branching apicomplexans. Accordingly, a robust inference of gregarine phylogeny is crucial to any interpretation of apicomplexan evolution, but molecular sequences from gregarines are restricted to a small number of small-subunit (SSU) rDNA sequences from derived taxa. This work examines the usefulness of SSU rDNA and β-tubulin sequences for inferring gregarine phylogeny. SSU rRNA genes from (Mingazzini) sp., Stein, Clopton and Dufour, as well as the β-tubulin gene from , were sequenced. The results of phylogenetic analyses of alveolate taxa using both genes were consistent with an early origin of gregarines and the putative ‘sister’ relationship between gregarines and , but neither phylogeny was strongly supported. In addition, two SSU rDNA sequences from unidentified marine eukaryotes were found to branch among the gregarines: one was a sequence derived from the haemolymph parasite of the giant clam, , and the other was a sequence misattributed to the foraminiferan . In all of our analyses, the SSU rDNA sequence from sp. clustered weakly with the apicomplexans, which is consistent with ultrastructural data. Altogether, the exact position of gregarines with respect to and other apicomplexans remains to be confirmed, but the congruence of SSU rDNA and β-tubulin trees with one another and with morphological data does suggest that further sampling of molecular data will eventually put gregarine diversity into a phylogenetic context.

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2003-01-01
2024-03-28
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