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Abstract
A terephthalate-assimilating bacterium was isolated from activated sludge collected from a domestic wastewater treatment plant in Japan by enrichment with terephthalate as sole carbon source. The isolate, designated strain T7T, was a Gram-negative, short rod-shaped micro-organism. A phylogenetic study based on 16S rRNA gene sequences indicated that strain T7T should be placed in the genus Delftia. A DNA–DNA hybridization value of 69 % was determined between strain T7T and Delftia acidovorans ATCC 15668T. Major cellular fatty acids of strain T7T were C16 : 0, C16 : 1 and C18 : 1. Substantial amounts of cyclopropanoic acid (C17 : 0), 3-OH C10 : 0, C12 : 0, C15 : 0 and C14 : 0 were also detected. The total DNA G+C content of strain T7T was 66·2 mol%. Strain T7T could utilize the following compounds as carbon sources: acetamide, β-alanine, citrate, d-fructose, glycerol, isobutyrate, isophthalate, d(−)-mannitol, maleate, malonate, phenylacetate, propionate, protocatechuate, terephthalate, d-tryptophan and l-tryptophan. Comparisons of phenotypic and genotypic characteristics with other known species belonging to the genus Delftia suggest that strain T7T represents a novel species, for which the name Delftia tsuruhatensis sp. nov. is proposed; strain T7T is the type strain (=IFO 16741T=ATCC BAA-554T).
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