RT Journal Article SR Electronic(1) A1 Liu, Zhu-Xiang A1 Chen, Jun A1 Tang, Shu-Kun A1 Zhang, Yu-Qin A1 He, Jian-Wu A1 Chen, Qi-Hui A1 Li, Wen-Jun A1 Chen, Yi-GuangYR 2011 T1 Jeotgalicoccus nanhaiensis sp. nov., isolated from intertidal sediment, and emended description of the genus Jeotgalicoccus JF International Journal of Systematic and Evolutionary Microbiology, VO 61 IS 9 SP 2029 OP 2034 DO https://doi.org/10.1099/ijs.0.022871-0 PB Microbiology Society, SN 1466-5034, AB A novel non-sporulating, non-motile, catalase- and oxidase-positive, strictly aerobic, Gram-positive-staining coccus, strain JSM 077023T, was isolated from an intertidal sediment sample collected from Naozhou Island in the South China Sea, China. Growth occurred in the presence of 0.5–25 % (w/v) NaCl [optimum, 2–5 % (w/v) NaCl] and at pH 5.5–10.5 (optimum, pH 7.0–8.0) and at 4–45 °C (optimum, 30–35 °C). The major amino acid constituents of the cell wall were alanine, glycine and lysine. The major cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0. The strain contained MK-7 and MK-6 as the predominant respiratory quinones and diphosphatidylglycerol, phosphatidylglycerol and an unidentified phospholipid as the polar lipids. The genomic DNA G+C content of strain JSM 077023T was 41.3 mol%. A phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 077023T should be assigned to the genus Jeotgalicoccus and was most closely related to the type strains of Jeotgalicoccus halotolerans (sequence similarity 99.0 %) and Jeotgalicoccus aerolatus (99.0 %), followed by Jeotgalicoccus coquinae (98.6 %) and Jeotgalicoccus psychrophilus (97.4 %). 16S rRNA gene sequence similarities of less than 97 % were observed with other species of the genus Jeotgalicoccus. Levels of DNA–DNA relatedness between strain JSM 077023T and the type strains of J. halotolerans, J. aerolatus, J. coquinae and J. psychrophilus ranged from 36.8 to 22.7 %. The combination of phylogenetic analysis, DNA–DNA relatedness values, phenotypic characteristics and chemotaxonomic data supported the suggestion that strain JSM 077023T represents a novel species of the genus Jeotgalicoccus, for which the name Jeotgalicoccus nanhaiensis sp. nov. is proposed. The type strain is JSM 077023T ( = DSM 23006T = KCTC 13714T). An emended description of the genus Jeotgalicoccus is also presented., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.022871-0