1887

Abstract

Two novel psychrophilic bacterial strains (ZF-6 and ZF-8) were isolated from the China No. 1 glacier. Polyphasic taxonomy using physiological and biochemical properties and phylogenetic analysis based on 16S rRNA gene sequences showed that the two isolates belonged to the genus , and that they were distinct from each other and also from the known species of this genus. Strains ZF-6 and ZF-8 are Gram-negative and both have an optimal growth temperature of 11 °C. Strain ZF-6 is able to grow at 0–20 °C, the G+C content of its genomic DNA is 34·4 mol% and the major fatty acids of ZF-6 are C 7 (17·7 %) and C 6 (12·7 %). Strain ZF-8 showed a strong ability to degrade organic macromolecules such as starch, CM-cellulose, pectin and chitin. Its DNA G+C content is 35·1 mol%, and the major fatty acids are C 7 (18·2 %) and C (9·9 %). Phylogenetic analysis based on 16S rDNA sequences indicated that ZF-6 and ZF-8 belong to the genus and represent two novel species. DNA–DNA hybridization also supported the status of the two new isolates. The names sp. nov. (type strain, ZF-6=AS 1.2749=JCM 11314) and sp. nov. (type strain, ZF-8=AS 1.2747=JCM 11313) are proposed for the two new isolates.

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2003-05-01
2024-03-29
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References

  1. Bergey D. H., Harrison F. C., Breed R. S., Hammer B. W., Huntoon F. M. 1923; Genus II. Flavobacterium gen. nov. In Bergey's Manual of Determinative Bacteriology pp 97–117 Baltimore: Williams & Wilkins;
    [Google Scholar]
  2. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. 1996; Cutting a Gordian knot: emended classification and description of the genus Flavobacterium , emended description of the family Flavobacteriaceae , and proposal of Flavobacterium hydatis nom. nov. (basonym Cytophaga aquatilis Strohl and Tait 1978. Int J Syst Bacteriol 46:128–148 [CrossRef]
    [Google Scholar]
  3. Christner B. C., Mosley-Thompson E., Thompson L. G., Zagorodnov V., Sandman K., Reeve J. N. 2000; Recovery and identification of viable bacteria immured in glacial ice. Icarus 144:479–485 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1993 phylip (Phylogeny Inference Package) version 3.75c University of Washington; Seattle, USA:
    [Google Scholar]
  5. Liu H., Xu Y., Ma Y., Zhou P. 2000; Characterization of Micrococcus antarcticus sp. nov., a psychrophilic bacterium from Antarctica. Int J Syst Evol Microbiol 50:715–719 [CrossRef]
    [Google Scholar]
  6. Hsu S. C., Lockwood J. L. 1975; Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Appl Microbiol 29:422–426
    [Google Scholar]
  7. Humphry D. R., George A., Black G. W., Cummings S. P. 2001; Flavobacterium frigidarium sp. nov., an aerobic, psychrophilic, xylanolytic and laminarinolytic bacterium from Antarctica. Int J Syst Evol Microbiol 51:1235–1243
    [Google Scholar]
  8. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp 21–132Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  9. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  10. Margesin R., Schinner F. 1994; Properties of cold-adapted microorganisms and their potential role in biotechnology. J Biotechnol 33:1–14 [CrossRef]
    [Google Scholar]
  11. McCammon S. A., Bowman J. P. 2000; Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov. and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [ Flavobacterium ] salegens as Salegentibacter salegens gen. nov., comb. nov. Int J Syst Evol Microbiol 501055–1063 [CrossRef]
    [Google Scholar]
  12. McCammon S. A., Innes B. H., Bowman J. P., Franzmann P. D., Dobson S. J., Holloway P. E., Skerratt J. H., Nichols P. D., Rankin L. M. 1998; Flavobacterium hibernum sp. nov., a lactose-utilizing bacterium from a freshwater Antarctic lake. Int J Syst Bacteriol 48:1405–1412 [CrossRef]
    [Google Scholar]
  13. Morita R. Y. 1975; Psychrophilic bacteria. Bacteriol Rev 39:144–167
    [Google Scholar]
  14. Russell N. J., Hamamoto T. 1998; Psychrophiles. In Extremophiles: Microbial Life in Extreme Environments pp 25–45Edited by Horikoshi K., Grant W. D. New York: Wiley;
    [Google Scholar]
  15. Sambrook J., Frisch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  16. Skerman V. B. D. 1967 A Guide to the Identification of Genera of Bacteria , 3rd edn. Baltimore: Williams & Wilkins;
    [Google Scholar]
  17. Sly L. I., Blackall L. L., Kraat P. C., Tian-Shen T., Sangkhobol V. 1986; The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. J Microbiol Methods 5:139–156 [CrossRef]
    [Google Scholar]
  18. Smibert R. M., Krieg N. R. 1981; Phenotypic characterization. In Manual of Methods for General Microbiology pp 611–654Edited by Gerhardt P., Murray R. G., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  19. Stackebrandt E., Koch C., Gvozdiak O., Schumann P. 1995; Taxonomic dissection of the genus Micrococcus : Kocuria gen. nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend. Int J Syst Bacteriol 45:682–692 [CrossRef]
    [Google Scholar]
  20. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acid Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  21. Tindall B. J., Ross H. N. M., Grant W. D. 1984; Natronobacterium gen. nov. and Natronococcus gen. nov. two new genera of haloalkaliphilic archaebacteria. Syst Appl Microbiol 5:41–57 [CrossRef]
    [Google Scholar]
  22. Van de Peer Y., De Wachter R. 1994; treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10:569–570
    [Google Scholar]
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