1887

Abstract

Five strains of thermotolerant lactic acid bacteria (G 12, G 22, G 35, G 43 and G 44) isolated from chicken faeces were characterized taxonomically. The strains were facultatively anaerobic, Gram-positive, catalase-negative, non-motile, non-spore-forming rods. They were heterofermentative lactobacilli that produced -lactic acid. Growth of the strains occurred at 45 °C but not at 15 °C. The optimum temperature for growth was 42 °C, as determined from the specific growth rate. The highest permissive temperatures for growth were 50 °C for strain G 35 and 48 °C for the other four strains. DNA G+C content of the strains was between 49 and 51 mol%. Complex fatty acid patterns of the strains showed the presence of C, C, sometimes C, C and C in the cell walls. Phylogenetic analysis of the 16S rRNA gene sequences of the five strains placed them in the / group, with as their closest relative (about 95 % sequence similarity). DNA–DNA hybridization data indicated that the thermotolerant strains were not . Taken together, the findings of this study show that the five strains isolated from chicken faeces represent a novel species within the genus , for which the name is proposed (G 35 =DSM 14792 =JCM 11425).

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2003-01-01
2024-03-29
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References

  1. Collins M. D., Rodrigues U., Ash C., Aguirre M., Farrow J. A. E., Martinez-Murcia A., Phillips B. A., Williams A. M., Wallbanks S. 1991; Phylogenetic analysis of the genus Lactobacillus and related lactic acid bacteria as determined by reverse transcriptase sequencing of 16S rRNA. FEMS Microbiol Lett 77:5–12 [CrossRef]
    [Google Scholar]
  2. Delcour J., Ferain T., Hols P. 2000; Advances in the genetics of thermophilic lactic acid bacteria. Curr Opin Biotechnol 11:497–504 [CrossRef]
    [Google Scholar]
  3. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the vax. Nucleic Acids Res 12:387–395 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  5. Fujisawa T., Shirasaka S., Watabe J., Mitsuoka T. 1984; Lactobacillus aviarius sp. nov.: a new species isolated from the intestine of chickens. Syst Appl Microbiol 5:414–420 [CrossRef]
    [Google Scholar]
  6. Fujisawa T., Benno Y., Yaeshima T., Mitsuoka T. 1992; Taxonomic study of the Lactobacillus acidophilus group, with recognition of Lactobacillus gallinarum sp. nov. and Lactobacillus johnsonii sp. nov. and synonymy of Lactobacillus acidophilus group A3 (Johnson et al . 1980) with the type strain of Lactobacillus amylovorus (Nakamura 1981). Int J Syst Bacteriol 42:487–491 [CrossRef]
    [Google Scholar]
  7. Gusils C., Palacios J., Gonzalez S., Oliver G. 1999; Lectin-like protein fractions in lactic acid bacteria isolated from chickens. Biol Pharm Bull 22:11–15 [CrossRef]
    [Google Scholar]
  8. Hanada S., Takaishi S., Matsuura K., Nakamura K. 2002; Roseiflexus castenholzii gen. nov., sp. nov. a thermophilic, filamentous, photosynthetic bacterium that lacks chlorosomes. Int J Syst Evol Microbiol 52:187–193
    [Google Scholar]
  9. Hoshi S., Sakata T., Mikuni K., Hashimoto H., Kimura S. 1994; Galactosylsucrose and xylosylfructoside alter digestive tract size and concentrations of cecal organic acids in rats fed diets containing cholesterol and cholic acid. J Nutr 124:52–60
    [Google Scholar]
  10. Jin L. Z., Ho Y. W., Abdullah N., Jalaludin S. 1998; Acid and bile tolerance of Lactobacillus isolated from chicken intestine. Lett Appl Microbiol 27:183–185 [CrossRef]
    [Google Scholar]
  11. Johnson J. L. 1973; Use of nucleic-acid homologies in the taxonomy of anaerobic bacteria. Int J Syst Bacteriol 23:308–315 [CrossRef]
    [Google Scholar]
  12. Kandler O., Weiss N. 1986; Genus Lactobacillus Beijerinck 1901, 212AL. In Bergey ' s Manual of Systematic Bacteriology . vol 2 pp 1209–1234Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
  13. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  14. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  15. Mori K., Yamazaki K., Ishiyama T., Katsumata M., Kobayashi K., Kawai Y., Inoue N., Shinano H. 1997; Comparative sequence analyses of the genes coding for 16S rRNA of Lactobacillus casei -related taxa. Int J Syst Bacteriol 47:54–57 [CrossRef]
    [Google Scholar]
  16. Otsuka M., Okada S., Uchimura T., Komagata K. 1994; A simple method for the determination of stereoisomers of lactic acid by HPLC using an enantiomeric resolution column, and its application to identification of lactic acid bacteria. Seibutsu-Kogaku Kaishi 72:81–86
    [Google Scholar]
  17. Roos S., Karner F., Axelsson L., Jonsson H. 2000; Lactobacillus mucosae sp. nov., a new species with in vitro mucus-binding activity isolated from pig intestine. Int J Syst Evol Microbiol 50:251–258 [CrossRef]
    [Google Scholar]
  18. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  19. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  20. Tannock G. W. 1999; Introduction. In Probiotics A Critical Review pp 1–4Edited by Tannock G. W. Norfolk: Horizon Scientific Press;
    [Google Scholar]
  21. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  22. Wayne L. G., Brenner D. J., Colwell R. R.9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
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