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Abstract

The term ‘flexispira’ refers to micro-organisms with a particular morphology: fusiform-shaped with helical periplasmic fibrils and bipolar tufts of sheathed flagella. Two flexispira taxa have been formally named, and , a third named species is and eight additional 16S rRNA sequence-based flexispira taxa have been described by Dewhirst . ( 50, 1781–1787, 2000) and given the provisional designation sp. flexispira taxa 1–5, 7, 8 and 10. In the present study, seven gastric or intestinal flexispira isolates from seven Finnish pigs originating from different farms were characterized. Morphologically, all these porcine isolates had typical flexispira morphology. Analysis of the 16S rDNA sequences of five isolates showed that they were most closely related to the sequences of flexispira taxa 4 and 5 and (taxon 6), but less closely related to taxa 1–3 and 8, and . Phenotypic characterization, analysis of RFLPs of 16S and 23S rDNAs and SDS-PAGE profiles revealed that all of the porcine isolates, reference strains of flexispira taxa 1, 4 and 5 and the type strain of (ATCC 700114) had highly related characteristics that differed from those of the reference strains of taxa 2, 3 and 8 and . Furthermore, a high DNA–DNA binding rate was found, in dot-blot hybridization studies, between the Finnish porcine strains, taxa 1, 4 and 5 reference strains and ATCC 700114. In conclusion, polyphasic characterization of novel porcine flexispira isolates and previously described taxa 1, 4 and 5 reference strains showed that they all belong to a validly described species, , and that the taxonomy of known flexispiras is less complicated than proposed on the basis of 16S rDNA sequence analysis.

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2003-03-01
2024-03-29
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