1887

Abstract

A filamentous actinomycete, designated strain CCNWNQ 0016, was isolated from soil from a zinc and copper mine in Shaanxi province, north-western China. A polyphasic taxonomic study was carried out to establish the status of strain CCNWNQ 0016. Chemical and morphological properties of the isolate were similar to those of species of the genus . Analysis of the almost complete 16S rRNA gene sequence placed strain CCNWNQ 0016 in the genus where it formed a distinct phyletic line with recognized species of this genus. The strain exhibited highest sequence similarities to NBRC 15428 (98.8 %), NBRC 12874 (98.7 %) and NBRC 12842 (98.7 %). Furthermore, DNA–DNA hybridization studies between the novel isolate and these three strains showed relatedness values of 49.6±0.6, 47.2±0.9 and 46.9±1.1 %, respectively. A number of phenotypic properties also enabled the isolate to be differentiated from related species of the genus . Therefore, it is proposed that strain CCNWNQ 0016 (=K42 =ACCC 41871 =HAMBI 3107) should be classified as the type strain of a novel species in the genus , sp. nov. The maximum level of resistance to Zn for strain CCNWNQ 0016 is 35 mM.

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2011-03-01
2024-03-29
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