1887

Abstract

Strain NRRL B-23883 was isolated from retail ground beef as part of a study on the genetic diversity of . The strain was found to be a strictly anaerobic, Gram-positive coccus that was able to utilize peptone as a sole carbon source. Analysis of the 16S rRNA gene sequence revealed that the strain was closely related to species within the genera and , but it was substantially different from the closest recognized species by nearly 10 % sequence divergence. The strain was also found to be closely related (>99 % sequence similarity) to an uncultured bacterial strain that was sequenced from a 16S rRNA gene clone library constructed to characterize the bacterial community of faeces from a captive spotted hyena. Strain NRRL B-23883 shared the peptidoglycan type A4β, -Orn–-Glu with members of the genus . Further phenotypic analysis revealed that strain NRRL B-23883 was able to utilize glycyl -methionine as a sole carbon source, in contrast to other species of the genus . Therefore, it is proposed that the isolate represents a novel species, sp. nov.; the type strain is NRRL B-23883 ( = DSM 22461).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.024232-0
2011-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/8/1962.html?itemId=/content/journal/ijsem/10.1099/ijs.0.024232-0&mimeType=html&fmt=ahah

References

  1. Collins M. D., Lawson P. A., Willems A., Cordoba J. J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J. A. E. 1994; The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44:812–826 [View Article][PubMed]
    [Google Scholar]
  2. Ezaki T., Kawamura Y., Li N., Li Z. Y., Zhao L., Shu S. 2001; Proposal of the genera Anaerococcus gen. nov., Peptoniphilus gen. nov. and Gallicola gen. nov. for members of the genus Peptostreptococcus . Int J Syst Evol Microbiol 51:1521–1528[PubMed]
    [Google Scholar]
  3. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [View Article][PubMed]
    [Google Scholar]
  4. Holdeman L. V., Cato E. P., Moore W. E. C. 1977 Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University;
    [Google Scholar]
  5. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [View Article]
    [Google Scholar]
  6. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp. 115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  7. MacKenzie S. L. 1987; Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70:151–160[PubMed]
    [Google Scholar]
  8. Marchesi J. R., Sato T., Weightman A. J., Martin T. A., Fry J. C., Hiom S. J., Dymock D., Wade W. G. 1998; Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl Environ Microbiol 64:795–799[PubMed]
    [Google Scholar]
  9. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [View Article][PubMed]
    [Google Scholar]
  10. Murdoch D. A. 1998; Gram-positive anaerobic cocci. Clin Microbiol Rev 11:81–120[PubMed]
    [Google Scholar]
  11. Rooney A. P., Swezey J. L., Friedman R., Hecht D. W., Maddox C. W. 2006; Analysis of core housekeeping and virulence genes reveals cryptic lineages of Clostridium perfringens that are associated with distinct disease presentations. Genetics 172:2081–2092 [View Article][PubMed]
    [Google Scholar]
  12. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  13. Schleifer K. H., Seidl P. H. 1985; Chemical composition and structure of murein. In Chemical Methods in Bacterial Systematics pp. 201–215 Edited by Minnikin G. M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  14. Song Y., Liu C., Finegold S. M. 2007; Peptoniphilus gorbachii sp. nov., Peptoniphilus olsenii sp. nov. and Anaerococcus murdochii sp. nov. isolated from clinical specimens of human origin. J Clin Microbiol 45:1746–1752 [CrossRef]
    [Google Scholar]
  15. Song Y., Liu C., Finegold S. M. 2010; Peptoniphilus gorbachii sp. nov., Peptoniphilus olsenii sp. nov. and Anaerococcus murdochii sp. nov. In List of New Names and New Combinations Previously Effectively, but not Validly, Published, Validation List no. 134. Int J Syst Evol Microbiol 60:1477–1479 [CrossRef]
    [Google Scholar]
  16. Steer T., Collins M. D., Gibson G. R., Hippe H., Lawson P. A. 2001; Clostridium hathewayi sp. nov., from human faeces. Syst Appl Microbiol 24:353–357 [View Article][PubMed]
    [Google Scholar]
  17. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  18. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  19. Ueki A., Abe K., Suzuki D., Kaku N., Watanabe K., Ueki K. 2009; Anaerosphaera aminiphila gen. nov., sp. nov., a glutamate-degrading, Gram-positive anaerobic coccus isolated from a methanogenic reactor treating cattle waste. Int J Syst Evol Microbiol 59:3161–3167 [View Article][PubMed]
    [Google Scholar]
  20. Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K.-H., Whitman W. B. (editors) 2009 Bergey’s Manual of Systematic Bacteriology. The Firmicutes vol. 3 New York: Springer;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.024232-0
Loading
/content/journal/ijsem/10.1099/ijs.0.024232-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error