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Volume 62, Issue Pt_4

Description of sp. nov., an anaerobe that produces hydrogen from glucose, and emended description of the genus Free

Abstract

A novel anaerobic, moderately thermophilic, NaCl-requiring fermentative bacterium, strain OS1, was isolated from oil production water collected from Alaska, USA. Cells were Gram-negative, non-motile, non-spore-forming rods (1.7–2.7 × 0.4–0.5 µm). The G+C content of the genomic DNA of strain OS1 was 46.6 mol%. The optimum temperature, pH and NaCl concentration for growth of strain OS1 were 55 °C, pH 7 and 10 g l, respectively. The bacterium fermented -fructose, -glucose, maltose, -mannose, α-ketoglutarate, -glutamate, malonate, pyruvate, -tartrate, -asparagine, Casamino acids, -cysteine, -histidine, -leucine, -phenylalanine, -serine, -threonine, -valine, inositol, inulin, tryptone and yeast extract. When grown on -glucose, 3.86 mol hydrogen and 1.4 mol acetate were produced per mol substrate. Thiosulfate, sulfur and -cystine were reduced to sulfide, and crotonate was reduced to butyrate with glucose as the electron donor. 16S rRNA gene sequence analysis indicated that strain OS1 was related to (99.7 % similarity to the type strain), a member of the phylum . DNA–DNA hybridization between strain OS1 and DSM 13490 yielded 68 % relatedness. Unlike , strain OS1 fermented malonate, maltose, tryptone, -leucine and -phenylalanine, but not citrate, fumarate, lactate, -malate, glycerol, pectin or starch. The major cellular fatty acid of strain OS1 was iso-C (91 % of the total). Strain OS1 also contained iso-C 3-OH (3 %), which was absent from , and iso-C (2 %), which was absent from . On the basis of these results, strain OS1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is OS1 ( = DSM 22491  = ATCC BAA-1850). An emended description of the genus is also given.

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Funding
This study was supported by the:
  • US Department of Energy (Award DE-FG02-08ER64689)
© 2012 IUMS
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2012-04-01
2024-04-19
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Description of Anaerobaculum hydrogeniformans sp. nov., an anaerobe that produces hydrogen from glucose, and emended description of the genus Anaerobaculum
Int J Syst Evol Microbiol 62, 832 (2012); https://doi.org/10.1099/ijs.0.024349-0
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