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Volume 53, Issue 4

sp. nov., a novel moderate halophile Free

Abstract

A moderately halophilic, Gram-positive, spore-forming bacterium was isolated from surface saline soil of the Karaj region, Iran. The strain, designated MA-2, was strictly aerobic with rod-shaped cells that occurred singly, in pairs or short chains. It contained -orn–-Asp-type peptidoglycan and the major respiratory lipoquinone was MK-7. It was non-motile and had an ellipsoidal endospore located centrally or subterminally. Growth occurred at 10–49 °C and in the pH range 6·0–9·6. Strain MA-2 grew at salinities of 1–24 % (w/v) NaCl, showing optimal growth at 10 % (w/v). The DNA G+C content was 41·3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MA-2 was associated with rRNA group 1. The micro-organisms showing the closest phylogenetic relationship to strain MA-2 were and . On the basis of phenotypic and chemotaxonomic characteristics, 16S rRNA gene sequence analysis and DNA–DNA similarity data, it is proposed that strain MA-2 (=DSM 14948 =LMG 21515) should be placed in the genus as the type strain of a novel species, sp. nov.

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2003-07-01
2024-04-20
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Halobacillus karajensis sp. nov., a novel moderate halophile
Int J Syst Evol Microbiol 53, 1059 (2003); https://doi.org/10.1099/ijs.0.02448-0
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