RT Journal Article SR Electronic(1) A1 Scola, Bernard La A1 Birtles, Richard J. A1 Greub, Gilbert A1 Harrison, Timothy J. A1 Ratcliff, Rodney M. A1 Raoult, DidierYR 2004 T1 Legionella drancourtii sp. nov., a strictly intracellular amoebal pathogen JF International Journal of Systematic and Evolutionary Microbiology, VO 54 IS 3 SP 699 OP 703 DO https://doi.org/10.1099/ijs.0.02455-0 PB Microbiology Society, SN 1466-5034, AB A Legionella-like amoebal pathogen (LLAP), formerly named LLAP12T, was characterized on the basis of microscopic appearance, staining characteristics, growth in Acanthamoeba polyphaga at different temperatures, DNA G+C content, serological cross-reactivity and 16S rRNA and macrophage infectivity potentiator (mip) gene sequence analysis. LLAP12T was found to be a motile, Gram-negative bacterium that grew within cytoplasmic vacuoles in infected amoebae. The infecting bacteria induced lysis of their amoebal hosts and time taken to do so was dependent on incubation temperature. Recovery of LLAP12T from amoebae onto axenic media could not be achieved. Phylogenetic analysis of LLAP12T, based on 16S rRNA and mip gene sequence analysis, indicated that it lay within the radiation of the Legionellaceae and that it clustered specifically with Legionella lytica and Legionella rowbothamii. The divergence observed between LLAP12T and these two species was of a degree equal to, or greater than, that observed between other members of the family. In support of this delineation, LLAP12T was found not to cross-react serologically with any other Legionella species. The mip and 16S rRNA gene sequence-based analyses also indicated that LLAP12T was related very closely to two other previously identified LLAP isolates, LLAP4 and LLAP11. Taken together, these results support the proposal of LLAP12T as the type strain of Legionella drancourtii sp. nov., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.02455-0