1887

Abstract

A -like amoebal pathogen (LLAP), formerly named LLAP12, was characterized on the basis of microscopic appearance, staining characteristics, growth in at different temperatures, DNA G+C content, serological cross-reactivity and 16S rRNA and macrophage infectivity potentiator () gene sequence analysis. LLAP12 was found to be a motile, Gram-negative bacterium that grew within cytoplasmic vacuoles in infected amoebae. The infecting bacteria induced lysis of their amoebal hosts and time taken to do so was dependent on incubation temperature. Recovery of LLAP12 from amoebae onto axenic media could not be achieved. Phylogenetic analysis of LLAP12, based on 16S rRNA and gene sequence analysis, indicated that it lay within the radiation of the and that it clustered specifically with and . The divergence observed between LLAP12 and these two species was of a degree equal to, or greater than, that observed between other members of the family. In support of this delineation, LLAP12 was found not to cross-react serologically with any other species. The and 16S rRNA gene sequence-based analyses also indicated that LLAP12 was related very closely to two other previously identified LLAP isolates, LLAP4 and LLAP11. Taken together, these results support the proposal of LLAP12 as the type strain of sp. nov.

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2004-05-01
2024-03-28
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References

  1. Adeleke A., Pruckler J., Benson R., Rowbotham T., Halablab M., Fields B. 1996; Legionella -like amebal pathogens – phylogenetic status and possible role in respiratory disease. Emerg Infect Dis 2:225–230 [CrossRef]
    [Google Scholar]
  2. Adeleke A. A., Fields B. S., Benson R. F. 8 other authors 2001; Legionella drozanskii sp. nov., Legionella rowbothamii sp. nov. and Legionella fallonii sp. nov.: three unusual new Legionella species. Int J Syst Evol Microbiol 51:1151–1160 [CrossRef]
    [Google Scholar]
  3. Ash C., Farrow J. A. E., Dorsch M., Stackebrandt E., Collins M. D. 1991; Comparative analysis of Bacillus anthracis , Bacillus cereus , and related species on the basis of reverse transcriptase sequencing of 16S rRNA. Int J Syst Bacteriol 41:343–346 [CrossRef]
    [Google Scholar]
  4. Barbaree J. M., Fields B. S., Feeley J. C., Gorman G. W., Martin W. T. 1986; Isolation of protozoa from water associated with a legionellosis outbreak and demonstration of intracellular multiplication of Legionella pneumophila . Appl Environ Microbiol 51:422–424
    [Google Scholar]
  5. Barker J., Brown M. R. 1994; Trojan horses of the microbial world: protozoa and the survival of bacterial pathogens in the environment. Microbiology 140:1253–1259 [CrossRef]
    [Google Scholar]
  6. Birtles R. J., Rowbotham T. J., Raoult D., Harrison T. G. 1996; Phylogenetic diversity of intra-amoebal legionellae as revealed by 16S rRNA gene sequence comparison. Microbiology 142:3525–3530 [CrossRef]
    [Google Scholar]
  7. Cirillo J. D., Cirillo S. L. G., Yan L., Bermudez L. E., Falkow S., Tompkins L. S. 1999; Intracellular growth in Acanthamoeba castellanii affects monocyte entry mechanisms and enhances virulence of Legionella pneumophila . Infect Immun 67:4427–4434
    [Google Scholar]
  8. Fallon R. J., Rowbotham T. J. 1990; Microbiological investigations into an outbreak of Pontiac fever due to Legionella micdadei associated with use of a whirlpool. J Clin Pathol 43:479–483 [CrossRef]
    [Google Scholar]
  9. Fox G. E., Wisotzkey J. D., Jurtshuk P. J. Jr 1992; How close is close: 16S rRNA sequence identity may not be sufficient to guarantee species identity. Int J Syst Bacteriol 42:166–170 [CrossRef]
    [Google Scholar]
  10. Fry N. K., Rowbotham T. J., Saunders N. A., Embley T. M. 1991; Direct amplification and sequencing of the 16S ribosomal DNA of an intracellular Legionella species recovered by amoebal enrichment from the sputum of a patient with pneumonia. FEMS Microbiol Lett 67:165–168
    [Google Scholar]
  11. Harrison T. G., Taylor A. G. 1988; Identification of legionellae by serological methods. In A Laboratory Manual for Legionella pp  57–68 Edited by Harrison T. G., Taylor A. G. London: Wiley;
    [Google Scholar]
  12. Horn M., Harzenetter M. D., Linner T., Schmid E. N., Müller K.-D., Michel R., Wagner M. 2001; Members of the Cytophaga - Flavobacterium - Bacteroides phylum as intracellular bacteria of acanthamoebae: proposal of ‘ Candidatus Amoebophilus asiaticus’. Environ Microbiol 3:440–449 [CrossRef]
    [Google Scholar]
  13. La Scola B., Fenollar F., Fournier P.-E., Altwegg M., Mallet M.-N., Raoult D. 2001; Description of Tropheryma whipplei gen. nov., sp. nov., the Whipple's disease bacillus. Int J Syst Evol Microbiol 51:1471–1479
    [Google Scholar]
  14. Ratcliff R. M., Lanser J. A., Manning P. A., Heuzenroeder M. W. 1998; Sequence-based classification scheme for the genus Legionella targeting the mip gene. J Clin Microbiol 36:1560–1567
    [Google Scholar]
  15. Rowbotham T. J. 1980; Preliminary report on the pathogenicity of Legionella pneumophila for freshwater and soil amoebae. J Clin Pathol 33:1179–1183 [CrossRef]
    [Google Scholar]
  16. Rowbotham T. J. 1983; Isolation of Legionella pneumophila from clinical specimens via amoebae, and the interaction of those and other isolates with amoebae. J Clin Pathol 36:978–986 [CrossRef]
    [Google Scholar]
  17. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
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