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Volume 61, Issue 8

Taxonomy, ontogeny and molecular phylogeny of spec. nov. (Ciliophora, Hypotrichida) from the Yellow Sea, China Free

Abstract

The morphology, morphogenesis and molecular phylogeny of the marine ciliated protozoan spec. nov., isolated from mollusc-farming waters of the Yellow Sea, Qingdao, PR China, were investigated using microscopic observations of live and protargol-impregnated specimens and by small subunit rRNA gene sequence analysis. The novel species could be distinguished by the following features: an elongated elliptical body, size 80–160 µm × 30–50 µm; an adoral zone consisting of about 36 membranelles; three frontal, one parabuccal, one buccal, two frontoterminal and usually two pretransverse ventral cirri; 10–13 transverse cirri; a midventral complex composed of 12–17 pairs of cirri only, terminating in posterior 1/5; four or five dorsal kineties; two types of colourless cortical granules; four to nine moniliform macronuclear nodules and one to three micronuclei, and a contractile vacuole positioned at mid-body. Hitherto, the ontogenesis of the genus has been regarded as rather diverse, which was confirmed by the morphogenetic processes of this novel species. The most noteworthy feature of was that the proter retained almost the entire parental adoral zone except for a few proximal membranelles that were renewed . The SSU rRNA gene sequence information clearly discriminated this isolate from its congeners. Molecular phylogenetic analyses demonstrated, with high statistical support, that branched as a sister lineage to the – clade and hence belongs to the core part of the order Urostylida.

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Funding
This study was supported by the:
  • Natural Science Foundation of China (Award 40976075)
  • Marie Curie Incoming International Fellowship
  • 7th European Community Framework Program
  • Center of Excellence in Biodiversity, King Saud University
  • 111 Project (Award B08049)
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2024-04-20
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Taxonomy, ontogeny and molecular phylogeny of Anteholosticha marimonilata spec. nov. (Ciliophora, Hypotrichida) from the Yellow Sea, China
Int J Syst Evol Microbiol 61, 2000 (2011); https://doi.org/10.1099/ijs.0.024638-0
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