RT Journal Article SR Electronic(1) A1 Belduz, Ali Osman A1 Dulger, Sabriye A1 Demirbag, ZihniYR 2003 T1 Anoxybacillus gonensis sp. nov., a moderately thermophilic, xylose-utilizing, endospore-forming bacterium JF International Journal of Systematic and Evolutionary Microbiology, VO 53 IS 5 SP 1315 OP 1320 DO https://doi.org/10.1099/ijs.0.02473-0 PB Microbiology Society, SN 1466-5034, AB Seven closely related xylanolytic, thermophilic bacilli were isolated from mud and water samples from the Gonen and Diyadin hot springs, respectively located in the Turkish provinces of Balikesir and Agri. On the basis of morphology and biochemical characteristics, one of the isolates, designated strain G2T, was studied further. Strain G2T is a xylanolytic, sporulating, Gram-positive, rod-shaped bacterium. The isolate is a thermophilic (optimum temperature for growth, 55–60 °C), facultative anaerobe that grows on a wide range of carbon sources, including glucose, starch, xylose and mannitol. It expressed a high level of xylose isomerase activity on xylose and also on glucose. 16S rRNA gene sequence analysis showed that this isolate resembled Anoxybacillus flavithermus DSM 2641T (>97 % similarity), but 16S–23S rDNA internally transcribed spacer polymorphism PCR showed variation between DSM 2641T and isolate G2T. However, it is also known that analysis of 16S rRNA gene sequences may be insufficient to distinguish between some species. In DNA–DNA hybridization, thermophilic isolate G2T showed relatedness of 53·4 % to A. flavithermus and about 45·0 % to Anoxybacillus pushchinoensis, indicating that it is distinct at the species level. On the basis of the evidence presented, it is proposed that strain G2T (=NCIMB 13933T=NCCB 100040T) be designated as the type strain of Anoxybacillus gonensis sp. nov., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.02473-0