RT Journal Article SR Electronic(1) A1 Dhanjal, Soniya A1 Kaur, Ishwinder A1 Korpole, Suresh A1 Schumann, Peter A1 Cameotra, Swaranjit Singh A1 Pukall, Rüdiger A1 Klenk, Hans-Peter A1 Mayilraj, ShanmugamYR 2011 T1 Agrococcus carbonis sp. nov., isolated from soil of a coal mine JF International Journal of Systematic and Evolutionary Microbiology, VO 61 IS 6 SP 1253 OP 1258 DO https://doi.org/10.1099/ijs.0.024745-0 PB Microbiology Society, SN 1466-5034, AB An actinobacterial strain, designated G4T, isolated from a coal mine was subjected to polyphasic taxonomic characterization. Cells were Gram-stain-positive, yellow-pigmented, non-motile and non-spore-forming cocci. This organism possessed a type B peptidoglycan with diaminobutyric acid as diagnostic diamino acid. The major respiratory quinones were MK-9, MK-10 and MK-11. The major fatty acids were anteiso-C15 : 0 (41.6  %) and anteiso-C17 : 0 (32.8 %). The predominant cellular polar lipids were diphosphatidylglycerol and phosphatidylglycerol. Cell wall sugars comprised galactose, glucose, ribose and rhamnose. 16S rRNA gene sequence analysis of strain G4T showed high similarity with Agrococcus baldri (98.9 %), Agrococcus citreus (97.8 %), Agrococcus jenensis (97.3 %) and Agrococcus terreus (97.0 %). Sequence similarity with the type strains of the other species of the genus Agrococcus was less than 97.0 %. The DNA–DNA relatedness of strain G4T with the type strains of Agrococcus baldri, Agrococcus citreus, Agrococcus jenensis and Agrococcus terreus was less than 70 %. On the basis of the physiological, biochemical and chemotaxonomic characteristics, strain G4T should be classified as the type strain of a novel species of the genus Agrococcus, for which the name Agrococcus carbonis sp. nov. is proposed. The type strain is G4T ( = MTCC 10213T  = DSM 22965T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.024745-0