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Volume 53, Issue 3

DNA–DNA hybridization study of species using genomic DNA macro-array analysis coupled to reverse genome probing Free

Abstract

The present study was aimed at simplifying procedures to delineate species and identify isolates based on DNA–DNA reassociation. DNA macro-arrays harbouring genomic DNA of reference strains of several species were produced. Labelled genomic DNA, hybridized to such an array, allowed multiple relative pairwise comparisons. Based on the relative DNA–DNA relatedness values, a complete data matrix was constructed and the ability of the method to discriminate strains belonging to different species was assessed. This simple approach led successfully to the discrimination of from , but also discriminated genomovars I and III, , , and . Present data showed a sufficient degree of congruence with previous DNA–DNA reassociation techniques. As part of a polyphasic taxonomic scheme, this straightforward approach is proposed to improve species definition, especially for application in the rapid screening necessary for large numbers of clinical or environmental isolates.

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Keyword(s): CF, cystic fibrosis , MF, microfluorimetry , NS1, nuclease S1 , R, hybridization signal ratio and SP, spectrophotometry
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2003-05-01
2024-04-18
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DNA–DNA hybridization study of Burkholderia species using genomic DNA macro-array analysis coupled to reverse genome probing
Int J Syst Evol Microbiol 53, 739 (2003); https://doi.org/10.1099/ijs.0.02483-0
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