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Abstract

A strictly aerobic, orange-pigmented and Gram-staining-negative bacterium, designated K17-16, was isolated from seawater of Gangjin Bay, Korea. Comparative 16S rRNA gene sequence analysis revealed that strain K17-16 was a member of the genus in the family and showed 94.0–95.6 % sequence similarity with the type strains of recognized species of the genus . The G+C content of the genomic DNA was 34.6 mol% and the major respiratory lipoquinone was MK-6. The major polar lipids detected were phosphatidylethanolamine, three unidentified amino-group-containing lipids and an unidentified aminophospholipid. The predominant cellular fatty acids were iso-C (15.4 %), C (12.4 %), summed feature 3 (comprising iso-C 2-OH and/or Cω7; 10.6 %), Cω6 (9.8 %) and iso-C 3-OH (8.6 %). On the basis of phenotypic and genotypic data, strain K17-16 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is K17-16 ( = KCTC 22729 = JCM 16152).

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2011-06-01
2024-03-28
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References

  1. Bernardet J.-F., Nakagawa Y. 2006; An introduction to the family Flavobacteriaceae . In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn. vol. 7 pp. 455–480 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. New York: Springer;
    [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  3. CLSI 2003 Performance Standards for Antimicrobial Disk Susceptibility Tests, 8th edn. Approved Standard M2–A8 Wayne, PA: Clinical Laboratory Standards Institute;
    [Google Scholar]
  4. Collins M. D., Jones D. 1980; Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48:459–470 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  6. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  7. Gosink J. J., Woese C. R., Staley J. T. 1998; Polaribacter gen. nov., with three new species, P. irgensii sp. nov., P. franzmannii sp. nov. and P. filamentus sp. nov., gas vacuolate polar marine bacteria of the CytophagaFlavobacterium–Bacteroides group and reclassification of ‘Flectobacillus glomeratus’ as Polaribacter glomeratus comb. nov.. Int J Syst Bacteriol 48:223–235 [View Article][PubMed]
    [Google Scholar]
  8. Hansen G. H., Sørheim R. 1991; Improved method for phenotypical characterization of marine bacteria. J Microbiol Methods 13:231–241 [View Article]
    [Google Scholar]
  9. Jeon Y. S., Chung H., Park S., Hur I., Lee J. H., Chun J. 2005; jPHYDIT: a JAVA-based integrated environment for molecular phylogeny of ribosomal RNA sequences. Bioinformatics 21:3171–3173 [View Article][PubMed]
    [Google Scholar]
  10. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp. 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  11. Komagata K., Suzuki K. 1987; Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
    [Google Scholar]
  12. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp. 115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  13. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [View Article][PubMed]
    [Google Scholar]
  14. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  15. Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Kalinovskaya N. I., Mikhailov V. V., Kim I. S., Bae K. S. 2005; Polaribacter butkevichii sp. nov., a novel marine mesophilic bacterium of the family Flavobacteriaceae . Curr Microbiol 51:408–412 [View Article][PubMed]
    [Google Scholar]
  16. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  17. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. 2001; Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov.. Int J Syst Evol Microbiol 51:1639–1652[PubMed] [CrossRef]
    [Google Scholar]
  18. Swofford D. L. 1998; Phylogenetic analysis using parsimony (paup), version 4.. Sunderland, MA: Sinauer Associates;
  19. Tindall B. J., Sikorski J., Simbert R. A., Kreig N. R. 2007; Phenotypic characterization and principles of comparative systematics. In Methods for General and Molecular Microbiology pp. 330–393 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  20. Yoon J.-H., Kang S.-J., Oh T.-K. 2006; Polaribacter dokdonensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 56:1251–1255 [View Article][PubMed]
    [Google Scholar]
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