1887

Abstract

A bacterial strain, SL-1, capable of degrading trichloroethene was isolated from a laboratory enrichment in the Department of Civil and Environmental Engineering, University of Washington, USA. The material in the enrichments was derived from a soil sample from Seattle, WA, USA. Strain SL-1 was capable of using phenol as a source of carbon and energy. Chemotaxonomic, morphological, physiological and phylogenetic analyses showed that strain SL-1 is a member of the genus . The ability of strain SL-1 to utilize phenol and degrade trichloroethene, as well as other phenotypic properties and the results from a 16S rRNA phylogenetic analysis, led to the proposal of a novel species, sp. nov. The type strain is SL-1 (=ATCC BAA-742=DSM 44698). Trichloroethene and other chloroethenes are major pollutants at many environmental sites, and has biodegradation properties that should be of interest to environmental microbiologists and engineers.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02488-0
2004-01-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/1/ijs540131.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02488-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
    [Google Scholar]
  2. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A. Struhl K. (editors) 1994 Current Protocols in Molecular Biology New York: Wiley;
    [Google Scholar]
  3. Bielefeldt A. R., Stensel H. D., Strand S. E. 1995; Cometabolic degradation of TCE and DCE without intermediate toxicity. J Environ Eng ASCE 121:791–797 [CrossRef]
    [Google Scholar]
  4. Bressler D. C., Leskiw B. K., Fedorak P. M. 1999; Biodegradation of benzothiophene sulfones by a filamentous bacterium. Can J Microbiol 45:360–368 [CrossRef]
    [Google Scholar]
  5. Coutts R. T., Hargesheimer E. E., Pasutto F. M. 1979; Gas chromatographic analysis of trace phenols by direct acetylation in aqueous solution. J Chromatogr 12:291–299
    [Google Scholar]
  6. Embley T. M., Smida J., Stackebrandt E. 1988; Reverse transcriptase sequencing of 16S ribosomal RNA from Faenia rectivirgula , Pseudonocardia thermophila and Saccharopolyspora hirsuta , three wall type IV actinomycetes which lack mycolic acids. J Gen Microbiol 134:961–966
    [Google Scholar]
  7. Ensley B. D. 1991; Biochemical diversity of trichloroethylene metabolism. Annu Rev Microbiol 45:283–299 [CrossRef]
    [Google Scholar]
  8. Evtushenko L. I., Akimov V. N., Dobritsa S. V., Taptykova S. D. 1989; A new species of actinomycete, Amycolata alni . Int J Syst Bacteriol 39:72–77 [CrossRef]
    [Google Scholar]
  9. Ewers J., Freier-Schröder D., Knackmuss H. J. 1990; Selection of trichloroethene (TCE) degrading bacteria that resist inactivation by TCE. Arch Microbiol 154:410–413
    [Google Scholar]
  10. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  11. Felsenstein J. 1995 phylip (Phylogeny Inference Package) version 3.573 University of Washington; Seattle, USA:
    [Google Scholar]
  12. Fetzner S. 1998; Bacterial dehalogenation. Appl Microbiol Biotechnol 50:633–657 [CrossRef]
    [Google Scholar]
  13. Gerritse J., Renard V., Visser J., Gottschal J. C. 1995; Complete degradation of tetrachloroethene by combining anaerobic dechlorinating and aerobic methanotrophic enrichment cultures. Appl Microbiol Biotechnol 43:920–928 [CrossRef]
    [Google Scholar]
  14. Gilbert D. G. 1999; SeqPup, a biological sequence editor and analysis program written in java. Available at http://iubio.bio.indiana.edu
  15. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca , Nocardia autotrophica , and the nocardin strain. Int J Syst Bacteriol 24:54–63 [CrossRef]
    [Google Scholar]
  16. Gouy M. 2000; NJplot. Laboratoire de Biométrie et Biologie Evolutive. Université Lyon: http://pbil.univ-lyon1.fr/software/njplot.html
  17. Gray J. P., Herwig R. P. 1996; Phylogenetic analysis of the bacterial communities in marine sediments. Appl Environ Microbiol 62:4049–4059
    [Google Scholar]
  18. Hall T. 2001 BioEdit Department of Microbiology, North Carolina State University;
    [Google Scholar]
  19. Huang Y., Wang L., Lu Z., Hong L., Liu Z., Tan G. Y. A., Goodfellow M. 2002; Proposal to combine the genera Actinobispora and Pseudonocardia in an emended genus Pseudonocardia , and description of Pseudonocardia zijingensis sp. nov. Int J Syst Evol Microbiol 52:977–982 [CrossRef]
    [Google Scholar]
  20. Juteau P., Larocque R., Rho D., LeDuy A. 1999; Analysis of the relative abundance of different types of bacteria capable of toluene degradation in a compost biofilter. Appl Microbiol Biotechnol 52:863–868 [CrossRef]
    [Google Scholar]
  21. Kesseler M., Dabbs E. R., Averhoff B., Gottschalk G. 1996; Studies on the isopropylbenzene 2,3-dioxygenase and the 3-isopropylcatechol 2,3-dioxygenase genes encoded by the linear plasmid of Rhodococcus erythropolis BD2. Microbiology 142:3241–3251 [CrossRef]
    [Google Scholar]
  22. Kohlweyer U., Thiemer B., Schrader T., Andreesen J. R. 2000; Tetrahydrofuran degradation by a newly isolated culture of Pseudonocardia sp. strain K1. FEMS Microbiol Lett 186:301–306 [CrossRef]
    [Google Scholar]
  23. Komagata K., Suzuki K.-I. 1987; Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–203
    [Google Scholar]
  24. Lee S.-B., Strand S. E., Stensel H. D. 2000; Sustained degradation of trichloroethylene in a suspended growth gas treatment reactor by an actinomycetes enrichment. Environ Sci Technol 34:3261–3268 [CrossRef]
    [Google Scholar]
  25. Lee S. D., Kim E. S., Min K.-L., Lee W. Y., Kang S.-O., Hah Y. C. 2001; Pseudonocardia kongjuensis sp. nov., isolated from a gold mine cave. Int J Syst Evol Microbiol 51:1505–1510
    [Google Scholar]
  26. Lee S. D., Kim E. S., Kang S.-O., Hah Y. C. 2002; Pseudonocardia spinosispora sp. nov., isolated from Korean soil. Int J Syst Evol Microbiol 52:1603–1608 [CrossRef]
    [Google Scholar]
  27. Maidak B. L., Cole J. R., Lilburn T. G. The RDP-II (Ribosomal Database Project) 7 other authors 2001 Nucleic Acids Res 29:173–174 [CrossRef]
    [Google Scholar]
  28. MIDI 1993 Microbial Identification System Operational Manual Newark, NJ: MIDI;
    [Google Scholar]
  29. Nelson M. J., Montgomery S. O., Mahaffey W. R., Pritchard P. H. 1987; Biodegradation of trichloroethylene and involvement of an aromatic biodegradative pathway. Appl Environ Microbiol 53:949–954
    [Google Scholar]
  30. Nelson M. J., Montgomery S. O., Pritchard P. H. 1988; Trichloroethylene metabolism by microorganisms that degrade aromatic compounds. Appl Environ Microbiol 54:604–606
    [Google Scholar]
  31. Olsen G. J., Matsuda H., Hagström R., Overbeek R. 1994; fastDNAmL: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood. Comput Appl Biosci 10:41–48
    [Google Scholar]
  32. Reichert K., Lipski A., Pradella S., Stackebrandt E., Altendorf K. 1998; Pseudonocardia asaccharolytica sp. nov. and Pseudonocardia sulfidoxydans sp. nov., two new dimethyl disulfide-degrading actinomycetes and emended description of the genus Pseudonocardia . Int J Syst Bacteriol 48:441–449 [CrossRef]
    [Google Scholar]
  33. Saddler G. S., Tavecchia P., Lociuro S., Zanol M., Colombo L., Selva E. 1991; Analysis of madurose and other actinomycete whole cell sugars by gas chromatography. J Microbiol Methods 14:185–191 [CrossRef]
    [Google Scholar]
  34. Saeki H., Akira M., Furuhashi K., Averhoff B., Gottschalk G. 1999; Degradation of trichloroethene by a linear-plasmid-encoded alkene monooxygenase in Rhodococcus corallinus ( Nocardia corallina ) B-276. Microbiology 145:1721–1730 [CrossRef]
    [Google Scholar]
  35. Semprini L. 1995; In situ bioremediation of chlorinated solvents. Environ Health Perspect 103 (Suppl. 5):101–105 [CrossRef]
    [Google Scholar]
  36. Sullivan J. P., Dickinson D., Chase H. A. 1998; Methanotrophs, Methylosinus trichosporium OB3b, sMMO, and their application to bioremediation. Crit Rev Microbiol 24:335–373 [CrossRef]
    [Google Scholar]
  37. US Environmental Protection Agency 1991 Survey of Material-handling Technologies Used at Hazardous Waste Sites . National Technical Information Service order number PB91-921283, report number EPA/540/2-91/010 Washington, DC: US Government Printing Office;
    [Google Scholar]
  38. Wackett L. P., Brusseau G. A., Householder S. R., Hanson R. S. 1989; Survey of microbial oxygenases: trichloroethylene degradation by propane-oxidizing bacteria. Appl Environ Microbiol 55:2960–2964
    [Google Scholar]
  39. Warwick S., Bowen T., McVeigh H., Embley T. M. 1994; A phylogenetic analysis of the family Pseudonocardiaceae and the genera Actinokineospora and Saccharothrix with 16S rRNA sequences and a proposal to combine the genera Amycolata and Pseudonocardia in an emended genus Pseudonocardia . Int J Syst Bacteriol 44:293–299 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02488-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02488-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error