Halogranum gelatinilyticum sp. nov. and Halogranum amylolyticum sp. nov., isolated from a marine solar saltern, and emended description of the genus Halogranum
Cui, Heng-Lin and Yang, Xin and Gao, Xia and Xu, Xue-Wei,, 61, 911-915 (2011),
doi = https://doi.org/10.1099/ijs.0.024976-0,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= Two extremely halophilic archaeal strains, designated TNN44T and TNN58T, were isolated from Tainan marine solar saltern near Lianyungang city, Jiangsu province, China. Cells of the two strains were pleomorphic and Gram-stain-negative; colonies were red-pigmented. Strains TNN44T and TNN58T were able to grow at 20–50 °C (optimum 37 °C for both), in the presence of 1.4–5.1 M NaCl (optimum 3.4–3.9 M NaCl) and at pH 5.5–9.0 (optimum pH 6.5–7.0); neither strain required Mg2+ for growth. Cells lysed in distilled water. On the basis of 16S rRNA gene sequence analysis, strains TNN44T and TNN58T were related closely to Halogranum rubrum RO2-11T (96.2 and 97.2 % similarity, respectively). The polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate (trace), and one major glycolipid and one minor glycolipid chromatographically identical to sulfated mannosyl glucosyl diether and mannosyl glucosyl diether, respectively; other trace unidentified lipids were also detected. The DNA G+C content of strains TNN44T and TNN58T was 64.0 and 62.0 mol%, respectively. The level of DNA–DNA relatedness between strains TNN44T and TNN58T was 37.2 %, and these two strains showed a low level of DNA–DNA relatedness with Halogranum rubrum RO2-11T (40.6 and 44.4 %, respectively). Two novel species of the genus Halogranum are proposed to accommodate these two strains, Halogranum gelatinilyticum sp. nov. (type strain TNN44T  = CGMCC 1.10119T  = JCM 16426T) and Halogranum amylolyticum sp. nov. (type strain TNN58T  = CGMCC 1.10121T  = JCM 16428T).,
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