1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 53, Issue 3

sp. nov., a novel keratinolytic soil actinomycete from Kuwait Free

Abstract

A keratinolytic, nocardioform actinomycete, strain D2, was isolated from Kuwait marsh soil by the bait technique using animal wool. Strain D2 was an aerobic, Gram-positive organism that produced light-grey aerial mycelium but no specific spore chains. 16S rDNA sequence analyses and chemotaxonomic markers were consistent with the classification of strain D2 in the genus , i.e. it had -diaminopimelic acid in its peptidoglycan, arabinose and galactose as its diagnostic sugars, the polar lipids phosphatidylinositol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine and diphosphatidylglycerol, menaquinone MK-9(H) and an iso-/anteiso-branched fatty acid pattern combined with 10-methyl-branched and 2-hydroxy-branched fatty acids. was the closest phylogenetic neighbour of strain D2, showing 99·4 % 16S rDNA sequence similarity with the novel strain. and strain D2 could be clearly separated from each other on the basis of their low DNA–DNA reassociation value (55·9 %). These data, together with its distinct physiological traits, led to the conclusion that strain D2 represented a novel species within the genus , for which the name (type strain D2=DSM 44409=NRRL B24117) is proposed.

  • Published Online:
SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02515-0
2003-05-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/53/3/ijs530871.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02515-0&mimeType=html&fmt=ahah

References

  1. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef]
     [Google Scholar]
  2. Chun J., Kim S. B., Oh Y. K.7 other authors 1999; Amycolatopsis thermoflava sp. nov., a novel soil actinomycete from Hainan Island, China. Int J Syst Bacteriol 49:1369–1373 [CrossRef]
     [Google Scholar]
  3. de Boer L., Dijkhuizen L., Grobben G., Goodfellow M., Stackebrandt E., Parlett J. H., Whitehead D., Witt D. 1990; Amycolatopsis methanolica sp. nov., a facultatively methylotrophic actinomycete. Int J Syst Bacteriol 40:194–204 [CrossRef]
     [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
     [Google Scholar]
  5. DeSoete G. 1983; A least squares algorithm for fitting additive trees to proximity data. Psychometrika 48:621–626 [CrossRef]
     [Google Scholar]
  6. Embley T. M. 1992; The family Pseudonocardiaceae . In The Prokaryotes , 2nd edn. pp 1188–1213Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer-Verlag;
     [Google Scholar]
  7. Escara J. F., Hutton J. R. 1980; Thermal stability and renaturation of DNA in dimethyl sulfoxide solutions: acceleration of the renaturation rate. Biopolymers 19:1315–1327 [CrossRef]
     [Google Scholar]
  8. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c Department of Genetics, University of Washington; Seattle, USA:
     [Google Scholar]
  9. Goodfellow M. 1971; Numerical taxonomy of some nocardioform bacteria. J Gen Microbiol 69:33–80 [CrossRef]
     [Google Scholar]
  10. Henssen A., Kothe H. W., Kroppenstedt R. M. 1987; Transfer of Pseudonocardia azurea and “ Pseudonocardia fastidiosa ” to the genus Amycolatopsis , with emended species description. Int J Syst Bacteriol 37:292–295 [CrossRef]
     [Google Scholar]
  11. Holt J. G., Krieg N. R., Sneath P. H. A., Stayley J. T., Williams S. T. 1994 Bergey's Manual of Determinative Bacteriology , 9th edn. Baltimore: Williams & Wilkins;
     [Google Scholar]
  12. Huss V. A. R., Festl H., Schleifer K.-H. 1983; Studies on the spectrometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
     [Google Scholar]
  13. Jahnke K.-D. 1992; Basic computer program for evaluation of spectroscopic DNA renaturation data from GILFORD System 2600 spectrometer on a PC/XT/AT type personal computer. J Microbiol Methods 15:61–73 [CrossRef]
     [Google Scholar]
  14. Jensen H. J. 1930; Decomposition of keratin by soil microorganisms. J Agric Sci 20:390–398 [CrossRef]
     [Google Scholar]
  15. Kane W. D. 1966; A new genus of Actinoplanaceae , Pilimelia , with a description of two species, Pilimelia terevasa and Pilimelia anulata . J Elisha Mitchell Sci Soc 82:220–230
     [Google Scholar]
  16. Kim B., Sahin N., Tan G. Y. A., Zakrzewska-Czerwinska J., Goodfellow M. 2002; Amycolatopsis eurytherma sp. nov., a thermophilic actinomycete isolated from soil. Int J Syst Evol Microbiol 52:889–894 [CrossRef]
     [Google Scholar]
  17. Kothe H. W., Vobis G., Kroppenstedt R. M., Henssen A. 1989; A taxonomic study of mycolateless, wall chemotype IV actinomycetes. Syst Appl Microbiol 12:61–69 [CrossRef]
     [Google Scholar]
  18. Kroppenstedt R. M. 1982; Separation of bacterial menaquinones by HPLC using reverse phase (RP-18) and a silver-loaded ion exchanger. J Liq Chromatogr 5:2359–2367 [CrossRef]
     [Google Scholar]
  19. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics no. 20SAB Technical Series pp 173–199Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
     [Google Scholar]
  20. Kroppenstedt R. M., Evtushenko L. I. 2002 The family Nocardiopsaceae In The Prokaryotes: an Evolving Electronic Resource for the Microbiological Community, 3rd edn (release 3.11).Edited by Dworkin M. New York: Springer; http://et.springer-ny.com:8080/prokPUB/index.htm
     [Google Scholar]
  21. Labeda D. P. 1995; Amycolatopsis coloradensis sp. nov., the avoparcin (LL-AV290)-producing strain. Int J Syst Bacteriol 45:124–127 [CrossRef]
     [Google Scholar]
  22. Lechevalier M. P., Prauser H., Labeda D. P., Ruan J.-S. 1986; Two new genera of nocardioform actinomycetes: Amycolata gen. nov. and Amycolatopsis gen. nov. Int J Syst Bacteriol 36:29–37 [CrossRef]
     [Google Scholar]
  23. Maidak B. L., Cole R. J., Parker C. T. Jr11 other authors 1999; A new version of the RDP (Ribosomal Database Project. Nucleic Acid Res 27:171–173 [CrossRef]
     [Google Scholar]
  24. Martin S. M., So V. 1969; Solubilization of autoclaved feathers and wool by myxobacteria. Can J Microbiol 15:1393–1397 [CrossRef]
     [Google Scholar]
  25. Miller L. T. 1982; Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16:584–586
     [Google Scholar]
  26. Minnikin D. E., Alshamaony L., Goodfellow M. 1975; Differentiation of Mycobacterium , Nocardia , and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88:200–204 [CrossRef]
     [Google Scholar]
  27. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117 [CrossRef]
     [Google Scholar]
  28. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athaly M., Schaal K. P., Parlett J. H. 1984; An integrated procedure for the extraction of isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
     [Google Scholar]
  29. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete. lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [CrossRef]
     [Google Scholar]
  30. Reichenbach H. 1992; The genus Lysobacter . In The Prokaryotes, 2nd edn. pp 3256–3275Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer-Verlag;
     [Google Scholar]
  31. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:1–6
     [Google Scholar]
  32. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
     [Google Scholar]
  33. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
     [Google Scholar]
  34. Vobis G., Schäfer D., Kothe H.-W., Renner B. 1986; Description of Pilimelia columellifera (ex Schäfer 1973), nom. rev. and Pilimelia columellifera subsp. pallida (ex Schäfer 1973), nom. rev. Syst Appl Microbiol 8:67–74 [CrossRef]
     [Google Scholar]
  35. Wayne L. G., Brenner D. J., Colwell R. R.9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
     [Google Scholar]
  36. Yassin A. F., Haggenei B., Budzikiewicz H., Schaal K. P. 1993; Fatty acid and polar lipid composition of the genus A mycolatopsis : application of fast atom bombardment-mass spectrometry to structure analysis of underivatized phospholipids. Int J Syst Bacteriol 43:414–420 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02515-0
Loading
Amycolatopsis keratiniphila sp. nov., a novel keratinolytic soil actinomycete from Kuwait
Int J Syst Evol Microbiol 53, 871 (2003); https://doi.org/10.1099/ijs.0.02515-0
/content/journal/ijsem/10.1099/ijs.0.02515-0
/content/journal/ijsem/10.1099/ijs.0.02515-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error